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PXD047228

PXD047228 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAffinity purification MS of human Ubiquitin-tagged RIT1
DescriptionHuman RIT1 was tagged with HA tag + N or C terminal ubiquitin (HA-Ubiqutin-RIT1 and HA-RIT1-Ubiqutin) and compared with empty vector (EV) or S35N/M90I RIT1 mutant (HA-RIT1 S35N/M90I). The constructs were expressed in human 293T cells and affinity purified using HA beads. RIT1 interacting proteins were compared by quantifying MS1 extracted chromatograms of peptides identified in conventional target-decoy database search.
HostingRepositoryPRIDE
AnnounceDate2024-07-09
AnnouncementXMLSubmission_2024-07-08_22:04:11.252.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAnatoly Urisman
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-24 12:26:04ID requested
12024-07-08 22:04:11announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Human, ubiquitin, RIT1
Contact List
Anatoly Urisman, MD PhD
contact affiliationUniversity of California San Francisco, Department of Pathology
contact emailanatoly.urisman@ucsf.edu
lab head
Anatoly Urisman
contact affiliationUniversity of California San Francisco (UCSF)
contact emailanatoly.urisman@ucsf.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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