PXD047228 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Affinity purification MS of human Ubiquitin-tagged RIT1 |
Description | Human RIT1 was tagged with HA tag + N or C terminal ubiquitin (HA-Ubiqutin-RIT1 and HA-RIT1-Ubiqutin) and compared with empty vector (EV) or S35N/M90I RIT1 mutant (HA-RIT1 S35N/M90I). The constructs were expressed in human 293T cells and affinity purified using HA beads. RIT1 interacting proteins were compared by quantifying MS1 extracted chromatograms of peptides identified in conventional target-decoy database search. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:49:14.333.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Anatoly Urisman |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-24 12:26:04 | ID requested | |
1 | 2024-07-08 22:04:11 | announced | |
⏵ 2 | 2024-10-22 06:49:14 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human, ubiquitin, RIT1 |
Contact List
Anatoly Urisman, MD PhD |
contact affiliation | University of California San Francisco, Department of Pathology |
contact email | anatoly.urisman@ucsf.edu |
lab head | |
Anatoly Urisman |
contact affiliation | University of California San Francisco (UCSF) |
contact email | anatoly.urisman@ucsf.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD047228 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047228
- Label: PRIDE project
- Name: Affinity purification MS of human Ubiquitin-tagged RIT1