PXD047206

PXD047206 is an original dataset announced via ProteomeXchange.

Title	The interactome of Complexin 4-SNARE complexes in mouse retina
Description	Complexin (Cplx)3 and Cplx4, SNARE-complex-regulators of the Cplx family, have been proposed to be involved in the light adaptation of ribbon synapses by limiting synaptic vesicle (SV) recruitment and fusion, but how this Cplx effect is exerted is unknown. Focusing on light adaptation of rod photoreceptor ribbon synapses, we first applied gel-based proteomics to FACS-sorted cone and rod photoreceptor cells to show that Cplx4 is the predominant Cplx in mouse rod photoreceptors, a finding that was confirmed by immunocytochemistry and RT-qPCR analyses. To uncover the functional network linking Cplx4 to light adaptation, we developed a quantitative proteomic screen to identify proteins that specifically interact with the Cplx4-SNARE complex at rod photoreceptor ribbon synapses. For affinity purification, detergent-extracted mouse retina lysates were incubated with Cplx peptide-coupled beads. Eluted proteins were subjected to in-solution digestion and analyzed by label-free quantification. We identified the G protein Transducin, a key molecule of the phototransduction cascade and known to translocate from the photoreceptor outer segment to the presynaptic terminal in light, as a component of the Cplx4-SNARE complex.
HostingRepository	PRIDE
AnnounceDate	2024-02-20
AnnouncementXML	Submission_2024-02-20_08:16:22.263.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-23 13:52:31	ID requested
⏵ 1	2024-02-20 08:16:22	announced

10.3389/FNMOL.2024.1308466;

submitter keyword: mouse retina, ribbon synapses, G protein, label-free quantification, Complexin 4, data-independent acquisition

Prof. Dr. Olaf Jahn
contact affiliation	Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen Germany
contact email	jahn@mpinat.mpg.de
lab head
Olaf Jahn
contact affiliation	Neuroproteomics Group, Max Planck Institute for Multidisciplinary Sciences, Göttingen
contact email	jahn@em.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD047206

PRIDE project URI

• PRIDE
  1. PXD047206
     1. Label: PRIDE project
     2. Name: The interactome of Complexin 4-SNARE complexes in mouse retina