PXD047204

PXD047204 is an original dataset announced via ProteomeXchange.

Title	Characterization of the pocket mutant of cyclin B1
Description	The APC complex was purified from insect cells and an in vitro phosphorylation purified WT cyclin B1 or pocket mutant cyclin B1 was performed. Phosphorylation sites were detected by mass spectrometry (in vitro data). WT cyclin B1 and mutant cyclin B1 was transfected in cells and the APC complex immunoprecipitated with an anti-APC3 antibody and binders identified by MS
HostingRepository	PRIDE
AnnounceDate	2025-04-03
AnnouncementXML	Submission_2025-04-03_09:43:34.821.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tanja Bange
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-23 13:25:05	ID requested
⏵ 1	2025-04-03 09:43:35	announced

Dataset with its publication pending

submitter keyword: phosphorylation, cyclin B1, pocket mutant cyclin B1, immunoprecipitation,APC complex

Tanja Bange
contact affiliation	Institute of Medical Psychology and Biomedical Center (BMC), Faculty of Medicine, LMU Munich, Germany
contact email	tanja.bange@med.uni-muenchen.de
lab head
Tanja Bange
contact affiliation	LMU Munich
contact email	tanja.bange@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD047204

PRIDE project URI

• PRIDE
  1. PXD047204
     1. Label: PRIDE project
     2. Name: Characterization of the pocket mutant of cyclin B1