PXD047188

PXD047188 is an original dataset announced via ProteomeXchange.

Title	Detection of candidate ovarian cancer protein biomarkers in sera using a multiplexed targeted mass spectrometry assay
Description	Ovarian cancer is the most lethal gynecologic malignancy in women, and high-grade serous ovarian cancer (HGSOC) is the most common subtype. Currently, no clinical test has been approved by the FDA to screen the general population for ovarian cancer. This underscores the critical need for the development of a robust methodology combined with novel technology to detect diagnostic biomarkers for HGSOC in the sera of women. Targeted mass spectrometry (MS) can be used to identify and quantify specific peptides/proteins in complex biological samples with high accuracy, sensitivity, and reproducibility. In this study, we sought to develop and conduct analytical validation of a multiplexed Tier 2 targeted MS parallel reaction monitoring (PRM) assay for the relative quantification of 23 putative ovarian cancer protein biomarkers in sera.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_10:38:57.912.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joohyun Ryu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-23 01:45:59	ID requested
⏵ 1	2024-01-26 10:38:59	announced

Ryu J, Boylan KLM, Twigg CAI, Evans R, Skubitz APN, Thomas SN, Quantification of putative ovarian cancer serum protein biomarkers using a multiplexed targeted mass spectrometry assay. Clin Proteomics, 21(1):1(2024) [pubmed]

10.1186/s12014-023-09447-4;

submitter keyword: high-grade serous ovarian cancer (HGSOC), insulin-like growth factor-binding protein 2 (IBP2), serum,Parallel Reaction Monitoring (PRM), diagnostic biomarkers

Stefani Thomas
contact affiliation	Department of Laboratory Medicine & Pathology, University of Minnesota
contact email	stefanit@umn.edu
lab head
Joohyun Ryu
contact affiliation	University of Minnesota
contact email	jryu@umn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD047188

PRIDE project URI

• PRIDE
  1. PXD047188
     1. Label: PRIDE project
     2. Name: Detection of candidate ovarian cancer protein biomarkers in sera using a multiplexed targeted mass spectrometry assay