PXD047183

PXD047183 is an original dataset announced via ProteomeXchange.

Title	A noncanonical role of roX RNAs in autosomal epigenetic repression
Description	Long noncoding RNAs known as roX (RNA on X) are crucial for male development in Drosophila, as their loss leads to male lethality from the late larval stages. While roX RNAs are recognized for their role in sex-chromosome dosage compensation, ensuring balanced expression of X-linked genes in both sexes, their potential influence on autosomal gene regulation remains unexplored. Our investigation reveals that roX RNAs not only govern the X chromosome but also target genes on autosomes that lack male-specific lethal (MSL) complex occupancy, together with Polycomb repressive complexes (PRCs). We observed that roX RNAs colocalize with MSL proteins on the X chromosome and PRC components on autosomes. Intriguingly, loss of roX function reduces H4K16ac levels on the X chromosome and H3K27me3 levels on autosomes. Correspondingly, X-linked genes display reduced expression, whereas many autosomal genes exhibit elevated expression upon roX gene deletion. Our findings propose a dual role for roX RNAs: activators of X-linked genes and repressors of autosomal genes, achieved through interactions with MSL and PRC complexes, respectively. This study uncovers the unconventional epigenetic repressive function of roX RNAs.
HostingRepository	PRIDE
AnnounceDate	2024-11-14
AnnouncementXML	Submission_2024-11-13_22:39:38.122.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	huipai Peng
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-22 18:28:46	ID requested
⏵ 1	2024-11-13 22:39:38	announced

Dataset with its publication pending

submitter keyword: ChIRP-MS,Polycomb repressive complexes,Long noncoding RNAs,roX

Qing Ma
contact affiliation	Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences.Shenzhen 518055，China
contact email	qing.ma@siat.ac.cn
lab head
huipai Peng
contact affiliation	CAS Key Laboratory for Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
contact email	hp.peng@siat.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047183
     1. Label: PRIDE project
     2. Name: A noncanonical role of roX RNAs in autosomal epigenetic repression