PXD047172

PXD047172 is an original dataset announced via ProteomeXchange.

Title	Proteomic approach during the induction of somatic embryo-genesis in Coffea canephora
Description	This research seeks to contribute to our understanding of the primary cellular pathways involved in the induction of SE in C. canephora. The process of our model consists of two stages: 1) preconditioning in MS medium with auxin (NAA) and cytokinin (KIN); and 2) induction in Yasuda liquid medium added with cytokinin (BA). Therefore, in this study, we analyzed different days of the SE induction process using shotgun label-free quantitative proteomics. 1630 proteins were found among different sampling days of the process, of which, the majority were up-accumulated during the induction stage. We found that some of the most enriched pathways during the process were the biosynthesis of amino acids and secondary metabolites. Eighteen proteins were found related to auxin homeostasis and two to cytokinin metabolism, such as ABC, BIG, ILR, LOG, and ARR, among others. Ten proteins and transcription factors related to SE were also identified, like SERK1, SKP1, nuclear transcription factor Y, MADS-box and calreticulin,; and 19 related to other processes of plant development, among which the 14-3-3 and PP2A proteins stand out.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_07:03:18.433.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047172
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	ANA QUINTANA
SpeciesList	scientific name: Coffea canephora; NCBI TaxID: 49390;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-22 07:08:01	ID requested
⏵ 1	2024-01-26 07:03:19	announced

Quintana-Escobar AO, Boj, ó, rquez-Vel, á, zquez E, Ruiz-May E, Loyola-Vargas VM, . Plants (Basel), 12(24):(2023) [pubmed]

10.6019/PXD047172;

10.3390/plants12244095;

submitter keyword: Coffea canephora, plant tissue culture, mass spectrometry, somatic embryogenesis

Victor Manuel Loyola Vargas
contact affiliation	Centro de Investigacion Cientifica de Yucatan
contact email	odetth@gmail.com
lab head
ANA QUINTANA
contact affiliation	CICY
contact email	odetth@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD047172

PRIDE project URI

• PRIDE
  1. PXD047172
     1. Label: PRIDE project
     2. Name: Proteomic approach during the induction of somatic embryo-genesis in Coffea canephora