PXD047169

PXD047169 is an original dataset announced via ProteomeXchange.

Title	Mustn1 is a smooth muscle cell-secreted micropeptide that modulates skeletal muscle extracellular matrix composition
Description	We show that Mustn1 (Musculoskeletal embryonic nuclear protein 1, also known as Mustang) is highly expressed in skeletal muscle during the early stages of hindlimb reloading. Mustn1 expression is transiently elevated in mouse and human skeletal muscle in response to intense exercise, resistance exercise, or injury. We find that Mustn1 expression is highest in smooth muscle-rich tissues, followed by skeletal muscle fibers. Muscle from heterozygous Mustn1-deficient mice exhibit differences in gene expression related to the extracellular matrix and cell adhesion, compared to wild-type littermates. Mustn1-deficient mice have normal muscle and aorta function and whole-body glucose metabolism. Loss of Mustn1 in vascular smooth muscle cells does not affect their proliferative or migratory functions. We show that Mustn1 can be secreted from smooth muscle cells, and that it is present in arterioles of the muscle microvasculature and in muscle interstitial fluid, in particular during the hindlimb reloading phase. Proteomics analysis of muscle from Mustn1-deficient mice confirms differences in extracellular matrix composition, and female mice display higher collagen content after chemically induced muscle injury compared to wild-type littermates.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:06.693.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonathan Van Vranken
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-22 05:37:43	ID requested
1	2024-05-23 00:53:19	announced
⏵ 2	2024-10-22 06:42:11	announced	2024-10-22: Updated project metadata.

Ducommun S, Jannig PR, Cervenka I, Murgia M, Mittenb, ü, hler MJ, Chernogubova E, Dias JM, Jude B, Correia JC, Van Vranken JG, Ocana-Santero G, Porsmyr-Palmertz M, McCann Haworth S, Mart, í, nez-Redondo V, Liu Z, Carlstr, ö, m M, Mann M, Lanner JT, Teixeira AI, Maegdefessel L, Spiegelman BM, Ruas JL, Mustn1 is a smooth muscle cell-secreted microprotein that modulates skeletal muscle extracellular matrix composition. Mol Metab, 82():101912(2024) [pubmed]

10.1016/j.molmet.2024.101912;

submitter keyword: skeletal muscle, extracellular matrix

Bruce Spiegelman
contact affiliation	Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02115, USA. Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
contact email	bruce_spiegelman@dfci.harvard.edu
lab head
Jonathan Van Vranken
contact affiliation	Harvard Medical School
contact email	van@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD047169

PRIDE project URI

• PRIDE
  1. PXD047169
     1. Label: PRIDE project
     2. Name: Mustn1 is a smooth muscle cell-secreted micropeptide that modulates skeletal muscle extracellular matrix composition