PXD047131

PXD047131 is an original dataset announced via ProteomeXchange.

Title	Propagation of a rapid cell-to-cell H2O2 signal over long distances in a monolayer of cardiomyocyte cells
Description	Cell-to-cell communication plays a cardinal role in the biology of multicellular organisms. H2O2 is an important cell-to-cell signaling molecule involved in the response of mammalian cells to wounding and other stimuli. We previously identified a signaling pathway that transmits wound-induced H2O2 cell-to-cell signals within minutes over long distances, measured in centimeters, in a monolayer of cardiomyocyte cells. Here we report that this long-distance H2O2 signaling pathway is accompanied by enhanced accumulation of cytosolic H2O2 and altered redox state in cells along its path. In addition, we show that it requires the production of superoxide, as well as the function of gap junctions, and that it is accompanied by significant changes in the abundance of hundreds of proteins in different cells along its path. Our findings highlight the existence of a unique rapid long-distance H2O2-dependent signaling pathway that could play an important role in different inflammatory responses, wound responses/healing, cardiovascular disease, and/or other diseases
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:22.229.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brian Mooney
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-21 10:59:39	ID requested
1	2024-05-24 01:43:11	announced
⏵ 2	2024-10-22 06:42:30	announced	2024-10-22: Updated project metadata.

10.1016/j.redox.2024.103069;

Fichman Y, Rowland L, Nguyen TT, Chen SJ, Mittler R, signal over long distances in a monolayer of cardiomyocyte cells. Redox Biol, 70():103069(2024) [pubmed]

submitter keyword: Hydrogen peroxide,Cell-to-cell, Superoxide, Signal transduction, Proteomics,Gap junction

Ron Mittler
contact affiliation	Plant Sciences University of Missouri
contact email	mittlerr@missouri.edu
lab head
Brian Mooney
contact affiliation	University of Missouri, Columbia
contact email	mooneyb@missouri.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047131
     1. Label: PRIDE project
     2. Name: Propagation of a rapid cell-to-cell H2O2 signal over long distances in a monolayer of cardiomyocyte cells