PXD047129

PXD047129 is an original dataset announced via ProteomeXchange.

Title	Focal clusters of peri-synaptic matrix contribute to activity-dependent plasticity and memory
Description	Experience-dependent learning depends on synaptic plasticity. Recent findings show that effective integration of novel salient information requires coordinated processes of homo- and hetero- synaptic plasticity onto neighboring dendritic branches. In this work, we hypothesized that activity-dependent remodeling of the peri-synaptic extracellular matrix (ECM) contributes to this mechanism. We show that clusters of the peri-synaptic ECM proteoglycans, containing 6- and 2,6-sulfated chondroitin sulfates recognized by CS56 antibody, emerge in response to sensory stimuli, showing temporal and spatial coincidence with dendritic spine plasticity. CS56 co-immunoprecipitation of synaptosomal proteins identified several CS56-carrying/binding synaptic molecules that are implicated in Ca2+ signaling, vesicles cycling and AMPA-receptor exocytosis, thus suggesting their pivotal role in long-term potentiation (LTP). Finally, we demonstrated that the attenuation of CS56 glycoepitopes in the CA1 hippocampal region, through the depletion of versican as one of its main carriers, impairs LTP and object location memory in adult mice. These findings show that specific ECM glycans regulates the molecular mechanisms underlying induction and consolidation of synaptic plasticity, confirming that experience-dependent refinement of the brain ECM plays a critical role in learning and memory.
HostingRepository	PRIDE
AnnounceDate	2024-05-10
AnnouncementXML	Submission_2024-05-10_07:14:50.387.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047129
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Manveen Sethi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-21 08:49:33	ID requested
⏵ 1	2024-05-10 07:14:51	announced

10.6019/PXD047129;

10.1016/j.celrep.2024.114112;

Chelini G, Mirzapourdelavar H, Durning P, Baidoe-Ansah D, Sethi MK, O'Donovan SM, Klengel T, Balasco L, Berciu C, Boyer-Boiteau A, McCullumsmith R, Ressler KJ, Zaia J, Bozzi Y, Dityatev A, Berretta S, Focal clusters of peri-synaptic matrix contribute to activity-dependent plasticity and memory in mice. Cell Rep, 43(5):114112(2024) [pubmed]

submitter keyword: synaptic plasticity, CS56 clusters,Extracellular matrix, versican., learning and memory

Joseph Zaia
contact affiliation	Boston University School of Medicine
contact email	jzaia@bu.edu
lab head
Manveen Sethi
contact affiliation	Boston University
contact email	msethi@bu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047129
     1. Label: PRIDE project
     2. Name: Focal clusters of peri-synaptic matrix contribute to activity-dependent plasticity and memory