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PXD047101

PXD047101 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSingle cell proteomics and epiproteomics of cancer cells treated with a histone deacetylate inhibitor
DescriptionNCI-H-358 cancer cells were treated with the histone deacetylase inhibitor mocetinostat or DMSO mock control. SIngle cells were isolated by FACs and were multiplexed using every other channel (c-Channels of the TMTPro reagent) a carrier channel of 75 control and 75 treated cells labeled with 135n were used for each experiment. Single cells were pseudo-randomized so that each following injection had an alternate mixture of control or treated cells as each label. Analysis was performed using an EvoSep system coupled to a TIMSTOF SCP. Cells were analyzed using 30SPD, 60SPD, 100SPD, 200SPD, 300SPD and 500SPD. After blank and carrier lanes, 7 cells were analyzed in each LCMS injection, allowing 210 cells, 420 cells, 700 cells, 1400 cells, 2100 cells and 3500 cells to be analyzed in a 24 hour day, respectively. Bruker .d files were converted to MGF and the reporter mass region was recalibrated to compensate for mass error. The resulting files were analyzed in Proteome Discoverer 2.4 and SCP-Viz.
HostingRepositoryMassIVE
AnnounceDate2023-11-20
AnnouncementXMLSubmission_2023-11-20_09:32:46.641.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterben
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListAcetyl; Dimethyl; Methyl
InstrumenttimsTOF fleX
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-20 08:05:31ID requested
12023-11-20 09:32:47announced
Publication List
no publication
Keyword List
submitter keyword: Histones, Single cell proteomics, single cell PTMs, HDAC proteomics
Contact List
Benjamin C Orsburn
contact affiliationJohns Hopkins
contact emailborsbur1@jhmi.edu
lab head
ben
contact affiliationThe Johns Hopkins School of Medicine
contact emailben_orsburn3@hotmail.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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