PXD047095 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic and transcriptomic analysis of selenium utilization in Methanococcus maripaludis |
Description | Methanococcus maripaludis utilizes selenocysteine-(Sec-) containing proteins (selenoproteins), mostly active in the organism’s primary energy metabolism, methanogenesis. Under selenium depletion, M. maripaludis employs a set of enzymes containing cysteine (Cys) instead of Sec. The genes coding for these Sec-/Cys-containing isoforms are the only genes known expression of which is influenced by the selenium status of the cell. Using quantitative proteomics and transcriptomics approx. 7% and 12%, respectively, of all genes/proteins were differentially expressed/synthesized in response to the selenium supply. Some of the genes identified involve methanogenesis, nitrogenase functions, and putative transporters. An increase of transcript abundance for putative transporters under selenium-depleted conditions indicated the organism’s effort to tap into alternative sources of selenium. Selenium sources M. maripaludis is known to utilize are selenite and dimethylselenide. To expand this list, a selenium responsive reporter strain was assessed with nine other, environmentally relevant selenium species. While some had a similar biological window as selenite, others were effectively utilized at lower concentrations. Conversely, selenate and seleno-amino acids were only utilized at unphysiologically high concentrations and two compounds were not utilized at all. To address the role of the selenium-regulated putative transporters in selenium transport, M. maripaludis mutant strains lacking one or two of the putative transporters were tested for the capability to utilize the different selenium species. Of the five putative transporters analyzed by loss-of-function mutagenesis, none appeared to be absolutely required for utilizing any of the selenium species tested, indicating they have redundant and/or overlapping specificities, or are not dedicated selenium transporters. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:34:42.334.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nico Jehmlich |
SpeciesList | scientific name: Methanococcus maripaludis; NCBI TaxID: 39152; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-20 04:00:06 | ID requested | |
1 | 2024-03-27 00:36:57 | announced | |
⏵ 2 | 2024-10-22 06:34:42 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Archaea,Selenium-dependent regulation, Methanococcus maripaludis, Proteome, Selenocysteine, Transcriptome |
Contact List
Nico Jehmlich |
contact affiliation | Helmholtz-Centre for Environmental Research - UFZ GmbH Department Molecular Systems Biology Permoserstrasse 15 04318 Leipzig, Germany |
contact email | nico.jehmlich@ufz.de |
lab head | |
Nico Jehmlich |
contact affiliation | Helmholtz-Centre for Environmental Research - UFZ |
contact email | nico.jehmlich@ufz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD047095 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047095
- Label: PRIDE project
- Name: Proteomic and transcriptomic analysis of selenium utilization in Methanococcus maripaludis