PXD047089

PXD047089 is an original dataset announced via ProteomeXchange.

Title	Human IgG1 (B-cell receptor) Fc peptide glycan analysis, LC-MS
Description	Since few data are available on glycans expressed by human BCRs, we aimed to analyze the Fc glycans of membrane-bound IgG after BCR capture and tryptic digestion by LC-MS. For this purpose, human B-cell lines from Burkitt Lymphoma (Ramos) expressing IgG1-BCRs in the presence or absence of Fc glycans (FcG) were analyzed. The B-cell lines were generated by transduction of the BCR-negative MDL-AID KO cell line with N(297) or mutant Q(297) IgG-BCRs. The BCR sequences used were derived from single-cell sorted human B cells isolated from patients with the autoimmune disease rheumatoid arthritis. Two BCRs were directed towards citrullinated antigens (2G9 and 3F3), while a third BCR was directed against tetanus toxoid (D2). In addition, we analyzed the Fc glycan profile of IgG secreted (sIgG) by human Ramos B cells for comparison.
HostingRepository	PRIDE
AnnounceDate	2023-12-11
AnnouncementXML	Submission_2023-12-11_01:35:55.414.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Theresa Kissel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	complex glycosylation
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-20 02:15:10	ID requested
⏵ 1	2023-12-11 01:35:55	announced

Dataset with its publication pending

submitter keyword: Human, B-cell receptor, IgG1 Fc peptide glycans, LC-MS

Rene E.M. Toes
contact affiliation	Department of Rheumatology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands
contact email	R.E.M.Toes@lumc.nl
lab head
Theresa Kissel
contact affiliation	Department of Rheumatology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands
contact email	T.Kissel@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD047089

PRIDE project URI

• PRIDE
  1. PXD047089
     1. Label: PRIDE project
     2. Name: Human IgG1 (B-cell receptor) Fc peptide glycan analysis, LC-MS