PXD047088

PXD047088 is an original dataset announced via ProteomeXchange.

Title	TMT-labeled differential proteomics study of the glycosyltransferase slr1064 mutant in cyanobacteria
Description	Glycosyltransferases (GTs) are enzymes that play important roles in various biological processes, such as photosynthesis, cell motility, exopolysaccharides, and lipid metabolism, by transferring sugars to different targets. However, the regulation of carbon metabolism by GTs in Synechocystis has not been reported yet. To identify novel GTs proteins involved in carbon metabolism, we performed a large-scale screening of GT gene-deletion mutants in Synechocystis. Our results revealed that the glycosyltransferase Slr1064, which is mainly distributed in the membrane compartment, is essential for the growth of Synechocystis under heterotrophic and mixotrophic conditions (HT and MT), but not under autotrophic conditions (AT). Integrated proteomic and metabolic analyses showed that Slr1064 regulates the Calvin cycle by modulating the levels of UDP-GlcNAc, which have been reported to mediate O-GlcNAc modification of Gap2. Further analyses demonstrated that the Δslr1064 mutant has a lower turnover rate of Gap2 under MT condition and shows dissociation of the PRK/GAPDH/CP12 complex under dark culture conditions. These findings provide new insights into the role of GTs in carbon metabolism in Synechocystis and expand our understanding of the regulatory mechanism of carbon metabolism in this important model organism.
HostingRepository	PRIDE
AnnounceDate	2024-05-28
AnnouncementXML	Submission_2024-05-27_19:07:39.540.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Longfa Fang
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-20 01:24:42	ID requested
⏵ 1	2024-05-27 19:07:39	announced

10.1111/NPH.19872;

submitter keyword: Turnover rate,Glycosyltransferases, UDP-GlcNAc, CP12, Calvin cycle

Longfa Fang
contact affiliation	professor of College of Pastoral Agriculture Science and Technology
contact email	fanglf@lzu.edu.cn
lab head
Longfa Fang
contact affiliation	18515055015
contact email	fanglf@lzu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047088
     1. Label: PRIDE project
     2. Name: TMT-labeled differential proteomics study of the glycosyltransferase slr1064 mutant in cyanobacteria