PXD047075

PXD047075 is an original dataset announced via ProteomeXchange.

Title	Comprehensive secretome and proteome analysis for human fetal brain self-organized organoids
Description	To understand whether the intrinsic capacity of the fetal brain in vitro self-organizes into organoids (FeBOs) to secrete a tissue-like “matrisome” is linked to the maintenance of cell-to-cell organization and integrity, we compared the secretome of intact FeBOs and FeBO-derived neurospheres. We also performed side-by-side comparative proteomic analysis of FeBOs and human fetal brain tissue to investigate if the extracellular matrix (ECM) production in the FeBOs resembled a proper tissue-like ECM niche. Moreover, we also included proteomic analysis of unguided pluripotent stem cell (PSC)-cerebral organoids and PSC-cortical spheroids to understand how the human brain tissue ECM-niche compared not only to the FeBOs but also to PSC-derived 3D brain models.
HostingRepository	PRIDE
AnnounceDate	2024-01-08
AnnouncementXML	Submission_2024-01-08_09:50:21.706.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ziliang Ma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-19 20:20:11	ID requested
⏵ 1	2024-01-08 09:50:22	announced

10.1016/J.CELL.2023.12.012;

submitter keyword: human fetal brain,Proteomics, tissue-derived organoids, LC-MS/MS

wei wu
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), 138648 Singapore, Singapore. Department of Pharmacy, National University of Singapore, 117543, Singapore, Singapore.
contact email	wu_wei@immunol.a-star.edu.sg
lab head
Ziliang Ma
contact affiliation	Utrecht University
contact email	z.ma@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD047075

PRIDE project URI

• PRIDE
  1. PXD047075
     1. Label: PRIDE project
     2. Name: Comprehensive secretome and proteome analysis for human fetal brain self-organized organoids