PXD047073
PXD047073 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of Interacting Proteins of mtAP2 Proteins Using IP and TurboID in Toxoplasma gondii |
Description | We employed anti-HA or FLAG immunoprecipitation (IP) combined with LC-MS/MS to identify interacting partners for endogenously C-terminal 6xHA-tagged mtAP2-(1/2/3) and endogenously C-terminal 3xFLAG-tagged mitochondrial ribosomal proteins (TguL4m, TgbL27m, and TguS5m). Additionally, we detected the protein abundance of LSU upon conditional knockdown of mtAP2-2 and mtAP2-3. Furthermore, we utilized the TurboID technology to identify the proximal proteins of three mtAP2s (mtAP2-1/2/3). |
HostingRepository | iProX |
AnnounceDate | 2023-11-17 |
AnnouncementXML | Submission_2024-10-24_23:32:25.839.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chaoyue Wang |
SpeciesList | scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-11-19 18:03:47 | ID requested | |
⏵ 1 | 2024-10-24 23:32:26 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Toxoplasma, mitoribosome, mtAP2, IP, TurboID |
Contact List
Xun Suo | |
---|---|
contact affiliation | China Agricultural University |
contact email | suoxun@cau.edu.cn |
lab head | |
Chaoyue Wang | |
contact affiliation | China Agricultural University |
contact email | 906513329@qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |