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PXD047073

PXD047073 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of Interacting Proteins of mtAP2 Proteins Using IP and TurboID in Toxoplasma gondii
DescriptionWe employed anti-HA or FLAG immunoprecipitation (IP) combined with LC-MS/MS to identify interacting partners for endogenously C-terminal 6xHA-tagged mtAP2-(1/2/3) and endogenously C-terminal 3xFLAG-tagged mitochondrial ribosomal proteins (TguL4m, TgbL27m, and TguS5m). Additionally, we detected the protein abundance of LSU upon conditional knockdown of mtAP2-2 and mtAP2-3. Furthermore, we utilized the TurboID technology to identify the proximal proteins of three mtAP2s (mtAP2-1/2/3).
HostingRepositoryiProX
AnnounceDate2023-11-17
AnnouncementXMLSubmission_2024-10-24_23:32:25.839.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChaoyue Wang
SpeciesList scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-19 18:03:47ID requested
12024-10-24 23:32:26announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Toxoplasma, mitoribosome, mtAP2, IP, TurboID
Contact List
Xun Suo
contact affiliationChina Agricultural University
contact emailsuoxun@cau.edu.cn
lab head
Chaoyue Wang
contact affiliationChina Agricultural University
contact email906513329@qq.com
dataset submitter
Full Dataset Link List
iProX dataset URI