PXD047071

PXD047071 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteomics on clade II N2O-reducer enrichments
Description	The majority of potent greenhouse gas nitrous oxide (N2O) emissions originate from microbially mediated reactions. The enzyme N2O reductase is the only known biological N2O sink and has evolved in two phylogenetically distinct lineages (clades I and II). Clade II is of particular interest for biotechnology as it is often associated with non-denitrifying N2O reducers. In this study, Laureni et al. investigated the environmental conditions that select for clade II. To do so, we enriched two N2O-respiring communities at low dilution rates, under both electron donor (acetate) and electron acceptor (N2O) limitations, in order to assess the impact of substrate affinity and N2O cytotoxicity on community assembly. We used a combination of genome-resolved metagenomics and shotgun metaproteomics to identify the taxonomy and metabolic potential of the steady-state community members. Corresponding author: Michele Laureni, contact: m.laureni@tudelft.nl
HostingRepository	PRIDE
AnnounceDate	2025-01-18
AnnouncementXML	Submission_2025-01-17_22:03:18.045.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Pabst
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-19 15:46:17	ID requested
⏵ 1	2025-01-17 22:03:18	announced

Dataset with its publication pending

submitter keyword: microbial proteomics, N2O,Shotgun proteomics, metaproteomics

Martin Pabst
contact affiliation	Department of Biotechnology, Delft University of Technology, The Netherlands.
contact email	m.pabst@tudelft.nl
lab head
Martin Pabst
contact affiliation	TUD
contact email	m.pabst@tudelft.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047071
     1. Label: PRIDE project
     2. Name: Shotgun proteomics on clade II N2O-reducer enrichments