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PXD047069

PXD047069 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleConsecutive digestion of archaeological skeletal proteomes with multiple proteases
DescriptionAncient skeletal proteomes are increasingly utilised for phylogenetic and evolutionary analysis. These proteomes are, however, often small and with low sequence coverage. We expand on previous observations which have shown that parallel digestion of Pleistocene skeletal proteomes increases proteome size and protein sequence coverage. Furthermore, we demonstrate that the consecutive digestion of a skeletal proteome using two proteases, particularly Glu-C or chymotrypsin followed by trypsin digestion, enables the recovery of alternative proteome components not reachable through trypsin digestion alone. The sequential utilisation of several proteases is a promising avenue for the study of highly degraded ancient proteomes for phylogenetic purposes.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:32:18.252.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZandra Fagernäs
SpeciesList scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationListmonohydroxylated residue; monohydroxylated proline; deamidated residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-19 14:35:55ID requested
12024-03-06 03:36:19announced
22024-10-22 06:32:18announced2024-10-22: Updated project metadata.
Publication List
10.1016/J.JPROT.2024.105143;
Keyword List
submitter keyword: Skeletal proteome,Palaeoproteomics, Archaeology, Protease
Contact List
Frido Welker
contact affiliationGlobe Institute, University of Copenhagen, Denmark
contact emailfrido.welker@sund.ku.dk
lab head
Zandra Fagernäs
contact affiliationGlobe Institute, University of Copenhagen
contact emailzandra.fagernas@sund.ku.dk
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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