PXD047069

PXD047069 is an original dataset announced via ProteomeXchange.

Title	Consecutive digestion of archaeological skeletal proteomes with multiple proteases
Description	Ancient skeletal proteomes are increasingly utilised for phylogenetic and evolutionary analysis. These proteomes are, however, often small and with low sequence coverage. We expand on previous observations which have shown that parallel digestion of Pleistocene skeletal proteomes increases proteome size and protein sequence coverage. Furthermore, we demonstrate that the consecutive digestion of a skeletal proteome using two proteases, particularly Glu-C or chymotrypsin followed by trypsin digestion, enables the recovery of alternative proteome components not reachable through trypsin digestion alone. The sequential utilisation of several proteases is a promising avenue for the study of highly degraded ancient proteomes for phylogenetic purposes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:32:18.252.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zandra Fagernäs
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	monohydroxylated residue; monohydroxylated proline; deamidated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-19 14:35:55	ID requested
1	2024-03-06 03:36:19	announced
⏵ 2	2024-10-22 06:32:18	announced	2024-10-22: Updated project metadata.

10.1016/J.JPROT.2024.105143;

submitter keyword: Skeletal proteome,Palaeoproteomics, Archaeology, Protease

Frido Welker
contact affiliation	Globe Institute, University of Copenhagen, Denmark
contact email	frido.welker@sund.ku.dk
lab head
Zandra Fagernäs
contact affiliation	Globe Institute, University of Copenhagen
contact email	zandra.fagernas@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD047069

PRIDE project URI

• PRIDE
  1. PXD047069
     1. Label: PRIDE project
     2. Name: Consecutive digestion of archaeological skeletal proteomes with multiple proteases