PXD047069 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Consecutive digestion of archaeological skeletal proteomes with multiple proteases |
Description | Ancient skeletal proteomes are increasingly utilised for phylogenetic and evolutionary analysis. These proteomes are, however, often small and with low sequence coverage. We expand on previous observations which have shown that parallel digestion of Pleistocene skeletal proteomes increases proteome size and protein sequence coverage. Furthermore, we demonstrate that the consecutive digestion of a skeletal proteome using two proteases, particularly Glu-C or chymotrypsin followed by trypsin digestion, enables the recovery of alternative proteome components not reachable through trypsin digestion alone. The sequential utilisation of several proteases is a promising avenue for the study of highly degraded ancient proteomes for phylogenetic purposes. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:32:18.252.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zandra Fagernäs |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Equus caballus (Horse); NCBI TaxID: 9796; |
ModificationList | monohydroxylated residue; monohydroxylated proline; deamidated residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-19 14:35:55 | ID requested | |
1 | 2024-03-06 03:36:19 | announced | |
⏵ 2 | 2024-10-22 06:32:18 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Skeletal proteome,Palaeoproteomics, Archaeology, Protease |
Contact List
Frido Welker |
contact affiliation | Globe Institute, University of Copenhagen, Denmark |
contact email | frido.welker@sund.ku.dk |
lab head | |
Zandra Fagernäs |
contact affiliation | Globe Institute, University of Copenhagen |
contact email | zandra.fagernas@sund.ku.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD047069 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047069
- Label: PRIDE project
- Name: Consecutive digestion of archaeological skeletal proteomes with multiple proteases