PXD047067
PXD047067 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A skin organoid-based infection platform identifies an inhibitor specific for HFMD |
| Description | The EV-A71 poses a serious threat to the health and lives of children. The EV-A71 can be transmitted by direct and indirect skin contact. Therefore, there is an urgent need to create novel skin models using human-derived cells to study the biology and pathogenesis of the virus and facilitate drug screening. Here, we used human pluripotent stem cells-derived skin organoids (hiPSC-SOs) as a model for EV-A71 infection and found that multiple cell types within the skin organoids, including epidermal cells, hair follicle cells, fibroblasts, and nerve cells, express EV-A71 receptors and are susceptible to EV-A71 infection. We elucidated the specific response of different cell types to EV-A71 and revealed that EV-A71 infection can degrade extracellular collagen and affect fibroblasts. We found that EV-A71 can mediate epidermal cell damage through autophagy and Integrin/Hippo-YAP/TAZ signaling pathways, thereby promoting hyperproliferation of progenitor cells. Based on this finding, we identified an autophagy-associated protein as a drug target of EV-A71 and discovered an EV-A71 replication inhibitor. Altogether, these data suggest that hiPSC-SOs can be used as an infectious disease model to study skin infectious diseases, providing a valuable resource for drug screening to identify candidate virus therapeutics. |
| HostingRepository | iProX |
| AnnounceDate | 2025-01-10 |
| AnnouncementXML | Submission_2025-01-09_21:42:55.185.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Qiyu Zhang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | monohydroxylated residue |
| Instrument | Q Exactive HF-X; NA sequence |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-11-19 08:40:25 | ID requested | |
| ⏵ 1 | 2025-01-09 21:42:55 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: human pluripotent stem cells, skin organoid, EV-A71 |
Contact List
| Ling Leng | |
|---|---|
| contact affiliation | Peking Union Medical College and Chinese Academy of Medical Sciences |
| contact email | lengling@pumch.cn |
| lab head | |
| Qiyu Zhang | |
| contact affiliation | Peking Union Medical College and Chinese Academy of Medical Sciences |
| contact email | gelosys@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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