PXD047063

PXD047063 is an original dataset announced via ProteomeXchange.

Title	Protein turnover dynamics of IAV-infected HeLa cells
Description	The abundance of a protein is defined by its continuous synthesis and degradation, a process known as protein turnover. Here, we systematically profiled the turnover of proteins in influenza A virus (IAV) infected cells using a pulse-chase SILAC-based approach combined with downstream statistical modeling. We identified 1414 virus-affected proteins with turnover changes (tVAPs) out of 7739 detected proteins. In particular, the affected proteins were involved in RNA transcription, splicing and nuclear transport, protein translation and stability, and energy metabolism. Many tVAPs are known IAV-interacting proteins that regulate virus propagation, such as KPNA6, PPP6C and POLR2A. Notably, our analysis identified novel IAV host and restriction factors, such as the splicing factor GPKOW, that exhibit significant turnover rate changes, while their total abundance is not affected. Overall, we show that protein turnover is a critical factor both for virus replication and antiviral defense.
HostingRepository	PRIDE
AnnounceDate	2025-04-09
AnnouncementXML	Submission_2025-04-09_06:07:18.414.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yiqi Huang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-19 02:49:38	ID requested
⏵ 1	2025-04-09 06:07:18	announced

10.1016/J.CELS.2024.09.004;

submitter keyword: IAV, pulse-chase, SILAC, HeLa, protein turnover,LC-MSMS

Andreas Pichlmair
contact affiliation	Institute of Virology - Viral Immunopathology, Faculty of Medicine, Technical University Munich
contact email	andreas.pichlmair@tum.de
lab head
Yiqi Huang
contact affiliation	TUM
contact email	yiqi.huang@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047063
     1. Label: PRIDE project
     2. Name: Protein turnover dynamics of IAV-infected HeLa cells