PXD047055 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | How processing affects marker peptide quantification – A comprehensive estimation on bovine material relevant for food and feed control |
Description | Processing food and feed challenges official control e.g. by modifying proteins, which leads to significant underestimation in targeted, MS-based protein quantification. Whereas numerous studies identified processing-induced changes on proteins in various combinations of matrices and processing conditions, studying their impact semi-quantitatively on specific protein sequences might unveil approaches to improve protein quantification accuracy. Thus, 335 post-translational modifications (e.g. oxidation, deamidation, carboxymethylation, Amadori, acrolein adduction) were identified by bottom-up proteomic analysis of 37 bovine materials relevant in food and feed (meat, bone, blood, milk) with varying processing degrees (raw, spray-dried, pressure-sterilized). To mimic protein recovery in a targeted analysis, peak areas of marker and reference peptides were compared to those of their modified versions, which revealed peptide-specific recoveries and variances across all samples. Detailed analysis suggests that incorporating two modified versions additionally to the unmodified marker may significantly improve quantification accuracy in targeted MS-based food and feed control in processed matrices. |
HostingRepository | PRIDE |
AnnounceDate | 2025-06-05 |
AnnouncementXML | Submission_2025-06-05_09:31:11.509.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD047055 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Uta M. Herfurth |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-18 04:38:23 | ID requested | |
⏵ 1 | 2025-06-05 09:31:11 | announced | |
Publication List
Keyword List
submitter keyword: ruminant, error-tolerant search, processing,proteomics, mass spectrometry, marker, post-translational modification (PTM), feed |
Contact List
Uta Maria |
contact affiliation | German Federal Institute for Risk Assessment Department Food Chemistry National Reference Laboratory for Animal Protein in Feed |
contact email | uta.herfurth@bfr.bund.de |
lab head | |
Uta M. Herfurth |
contact affiliation | German Federal Institute for Risk Assessment
National Reference Laboratory for Animal Proteins in Feed
Department Food Safety
Max-Dohrn-Straße 8-10, 10589 Berlin, Germany |
contact email | uta.herfurth@bfr.bund.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047055
- Label: PRIDE project
- Name: How processing affects marker peptide quantification – A comprehensive estimation on bovine material relevant for food and feed control