PXD047055

PXD047055 is an original dataset announced via ProteomeXchange.

Title	How processing affects marker peptide quantification – A comprehensive estimation on bovine material relevant for food and feed control
Description	Processing food and feed challenges official control e.g. by modifying proteins, which leads to significant underestimation in targeted, MS-based protein quantification. Whereas numerous studies identified processing-induced changes on proteins in various combinations of matrices and processing conditions, studying their impact semi-quantitatively on specific protein sequences might unveil approaches to improve protein quantification accuracy. Thus, 335 post-translational modifications (e.g. oxidation, deamidation, carboxymethylation, Amadori, acrolein adduction) were identified by bottom-up proteomic analysis of 37 bovine materials relevant in food and feed (meat, bone, blood, milk) with varying processing degrees (raw, spray-dried, pressure-sterilized). To mimic protein recovery in a targeted analysis, peak areas of marker and reference peptides were compared to those of their modified versions, which revealed peptide-specific recoveries and variances across all samples. Detailed analysis suggests that incorporating two modified versions additionally to the unmodified marker may significantly improve quantification accuracy in targeted MS-based food and feed control in processed matrices.
HostingRepository	PRIDE
AnnounceDate	2025-06-05
AnnouncementXML	Submission_2025-06-05_09:31:11.509.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047055
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Uta M. Herfurth
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-18 04:38:23	ID requested
⏵ 1	2025-06-05 09:31:11	announced

10.1016/J.FOODCHEM.2024.139768;

10.6019/PXD047055;

submitter keyword: ruminant, error-tolerant search, processing,proteomics, mass spectrometry, marker, post-translational modification (PTM), feed

Uta Maria
contact affiliation	German Federal Institute for Risk Assessment Department Food Chemistry National Reference Laboratory for Animal Protein in Feed
contact email	uta.herfurth@bfr.bund.de
lab head
Uta M. Herfurth
contact affiliation	German Federal Institute for Risk Assessment National Reference Laboratory for Animal Proteins in Feed Department Food Safety Max-Dohrn-Straße 8-10, 10589 Berlin, Germany
contact email	uta.herfurth@bfr.bund.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047055
     1. Label: PRIDE project
     2. Name: How processing affects marker peptide quantification – A comprehensive estimation on bovine material relevant for food and feed control