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PXD047055

PXD047055 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHow processing affects marker peptide quantification – A comprehensive estimation on bovine material relevant for food and feed control
DescriptionProcessing food and feed challenges official control e.g. by modifying proteins, which leads to significant underestimation in targeted, MS-based protein quantification. Whereas numerous studies identified processing-induced changes on proteins in various combinations of matrices and processing conditions, studying their impact semi-quantitatively on specific protein sequences might unveil approaches to improve protein quantification accuracy. Thus, 335 post-translational modifications (e.g. oxidation, deamidation, carboxymethylation, Amadori, acrolein adduction) were identified by bottom-up proteomic analysis of 37 bovine materials relevant in food and feed (meat, bone, blood, milk) with varying processing degrees (raw, spray-dried, pressure-sterilized). To mimic protein recovery in a targeted analysis, peak areas of marker and reference peptides were compared to those of their modified versions, which revealed peptide-specific recoveries and variances across all samples. Detailed analysis suggests that incorporating two modified versions additionally to the unmodified marker may significantly improve quantification accuracy in targeted MS-based food and feed control in processed matrices.
HostingRepositoryPRIDE
AnnounceDate2025-06-05
AnnouncementXMLSubmission_2025-06-05_09:31:11.509.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD047055
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterUta M. Herfurth
SpeciesList scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-18 04:38:23ID requested
12025-06-05 09:31:11announced
Publication List
10.1016/J.FOODCHEM.2024.139768;
10.6019/PXD047055;
Keyword List
submitter keyword: ruminant, error-tolerant search, processing,proteomics, mass spectrometry, marker, post-translational modification (PTM), feed
Contact List
Uta Maria
contact affiliationGerman Federal Institute for Risk Assessment Department Food Chemistry National Reference Laboratory for Animal Protein in Feed
contact emailuta.herfurth@bfr.bund.de
lab head
Uta M. Herfurth
contact affiliationGerman Federal Institute for Risk Assessment National Reference Laboratory for Animal Proteins in Feed Department Food Safety Max-Dohrn-Straße 8-10, 10589 Berlin, Germany
contact emailuta.herfurth@bfr.bund.de
dataset submitter
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Dataset FTP location
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