PXD047048

PXD047048 is an original dataset announced via ProteomeXchange.

Title	Therapeutic Potential of Eleutheroside E:Alleviates Cisplatin-Induced ototoxicity, inhibiting cochlear cells pyroptosis by down-regulating MAPK/ NF-κB /NLRP3 Signalling Pathway
Description	As one of the widely-used broad-spectrum anti-cancer drug, ototoxicity occurrence limited the usage of Cisplatin in clinical practice. At the same time, Eleutheroside E are found with kindny and never protection. However, the mechanism of the protection is remained unclear. In this study, 27 C57BL/6J mice were used and divided into Ctrl group with normal Saline injected, Cis and Cis + EE group with injection of Cis and Cis + EE peritoneally for Cisplatin-reduced ototoxicity modelling and intervention, then cochlears were dissected for proteomics. Network pharmacology to predict the target and biological mechanism of Eleutheroside E against cisplatin ototoxicity and Eleutheroside E might play an antagonistic role through the regulation of MAPK and pyrogenic signaling pathway. Compare with Cis group, Cis+EE group mice have a lower threshold value in ABR result and the group shows less cell pyroptosis than Cis group. Morphology results also indicated that Cis+EE group reduced space of spiral ganglion and the vascular structure was relatively clear than the Cis group. In addition, compared with Cis group, mRNA expression of NLRP3, GSDMD, ASC, Caspase-1, IL-1β and IL-18 genes in Cis+EE group decreased. The expression of p-JNK, p-ERK, P-P38, NF-κB P65, Cleaved Caspase-1, GSDMD was decreased, and the expression of IκB was increased. This study demonstrated that Eleutheroside E exerts an antagonistic effect on Cisplatin ototoxicity by down-regulating the activity of MAPK/NF-κB/NLRP3 signaling pathway, reducing the expression level of pyroptosis related proteins and inflammatory factors after cisplatin administration, and then inhibiting pyroptosis.
HostingRepository	iProX
AnnounceDate	2023-11-16
AnnouncementXML	Submission_2023-11-18_00:59:39.574.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yong Tang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-18 00:59:28	ID requested
⏵ 1	2023-11-18 00:59:39	announced

Dataset with its publication pending

submitter keyword: cisplatin, Eleutheroside E, ototoxicity, pyroptosis, inner ear

Yong Tang
contact affiliation	School of Clinical Medicine, Changchun University of Chinese Medicine
contact email	tangyong@ccucm.edu.cn
lab head
Yong Tang
contact affiliation	School of Clinical Medicine, Changchun University of Chinese Medicine
contact email	21102570671@stu.ccucm.edu.cn
dataset submitter

iProX dataset URI