PXD047032

PXD047032 is an original dataset announced via ProteomeXchange.

Title	NS7a of SADS-CoV promotes viral infection via inducing apoptosis to suppress Type III interferon production
Description	SADS-CoV is a newly discovered swine coronavirus with potential cross-species transmission risk. Although SADS-CoV-induced host cell apoptosis and attenuated innate immunity has been established, the relation between apoptosis and innate immunity remain obscure. Here, we demonstrate that infection of SADS-CoV induced apoptosis in vivo and in vitro, and that viral protein NS7a is mainly responsible for SADS-CoV-induced apoptosis in host cells. Furthermore, we found that NS7a interacted with apoptosis inducing factor mitochondria associated 1 (AIFM1) to activate caspase-3 via caspase-6 in SADS-CoV-infected cells, and enhanced SADS-CoV replication. Importantly, NS7a suppressed Poly(I:C)-induced expression of Type III interferon (IFN-λ) via activation caspase-3 to cleave IRF3, and caspase-3 inhibitor protects piglets against SADS-CoV infection in vivo. These findings reveal how SADS-CoV-induced apoptosis to inhibit innate immunity and provide a valuable clue to the development of effective drugs for the clinical control of SADS-CoV infection.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_12:11:09.439.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaowei Wang
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-16 21:33:50	ID requested
⏵ 1	2025-05-06 12:11:10	announced

10.1128/jvi.00317-24;

Wang X, Qiu W, Hu G, Diao X, Li Y, Li Y, Li P, Liu Y, Feng Y, Xue C, Cao Y, Xu Z, NS7a of SADS-CoV promotes viral infection via inducing apoptosis to suppress type III interferon production. J Virol, 98(5):e0031724(2024) [pubmed]

submitter keyword: viral replication,Swine acute diarrhea syndrome virus (SADS-CoV), NS7a, Apoptosis, Apoptosis inducing factor mitochondria associated 1 (AIFM1), IFN-λ

Zhichao Xu
contact affiliation	School of Life Science, Sun Yat-sen University, Guangzhou, 510006, China
contact email	xuzhich5@mail.sysu.edu.cn
lab head
Xiaowei Wang
contact affiliation	School of Life Science, Sun Yat-sen University
contact email	wangxw67@mail2.sysu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD047032

PRIDE project URI

• PRIDE
  1. PXD047032
     1. Label: PRIDE project
     2. Name: NS7a of SADS-CoV promotes viral infection via inducing apoptosis to suppress Type III interferon production