PXD047025

PXD047025 is an original dataset announced via ProteomeXchange.

Title	Analysis of protein biosynthesis errors in Candida albicans.
Description	Recent works show that protein mistranslation is widespread in nature and that both single cell or multicellular organisms can take advantage of it, by regulating its levels, under specific physiological and environmental conditions. In C. albicans, it leads to increased morphological and physiological phenotypic diversity of high adaptive potential, but the scope of such protein mistranslation is poorly understood due to technical difficulties in detecting and quantifying amino acid misincorporation events in complex proteomic samples. The phenomenon of mistranslation has been extensively studied in the leucine CUG codon, which has been reassigned to either serine or alanine, or ambiguously assigned to serine and leucine in several fungal species of the so called CTG clade. Serine-to-leucine (Ser→Leu) ambiguous decoding has been observed in Ascoidea asiatica, Candida maltosa, C. albicans and more recently, in the halotolerant yeast Debaryomyces hansenii. In C. albicans, CUG translation is facilitated by a hybrid tRNA(CAG)Ser that contains identity elements for both seryl-tRNA synthetase (SerRS) and leucyl-tRNA synthetase (LeuRS). Under normal physiological conditions the tRNA(CAG)Ser is mainly aminoacylated with Ser by the SerRS (appx 97%). We have developed and optimized mass spectrometry and bioinformatics pipelines capable of identifying low-level amino acid misincorporation events at the proteome level and determine codons error frequencies. We have also analysed the proteomic profile of an engineered C. albicans strain that exhibits high level of leucine misincorporation at protein CUG sites.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:57:49.849.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047025
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Inês Correia
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-16 09:14:45	ID requested
1	2024-09-09 04:15:51	announced
⏵ 2	2024-10-22 06:57:50	announced	2024-10-22: Updated project metadata.

10.1016/J.MCPRO.2024.100818;

10.6019/PXD047025;

submitter keyword: Candida albicans, proteogenomics, CUG ambiguity, mistranslation

Manuel Santos
contact affiliation	Institute of Biomedicine (iBiMED) and Department of Medical Sciences (DCM), University of Aveiro, Aveiro, Portugal. Multidisciplinary Institute of Ageing (MIA-Portugal), University of Coimbra, Coimbra, Portugal.
contact email	mansilvasantos@uc.pt
lab head
Inês Correia
contact affiliation	iBiMED - Institute of Biomedicine, University of Aveiro
contact email	inescorreia@ua.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD047025

PRIDE project URI

• PRIDE
  1. PXD047025
     1. Label: PRIDE project
     2. Name: Analysis of protein biosynthesis errors in Candida albicans.