PXD047014

PXD047014 is an original dataset announced via ProteomeXchange.

Title	Methodological aspects of µLC-MS/MS for wide-scale proteomic analysis of anthracycline-induced cardiomyopathy
Description	The effort to utilize microflow liquid chromatography for deep-scale proteomic analysis (µLC-MS/MS) is still growing. In this work, we utilized two-dimensional LC separation and peptide derivatization by tandem mass tag and assessed the capability of µLC-MS/MS to reveal protein changes associated with severe anthracycline-induced cardiomyopathy phenotype in comparison with nLC-MS/MS. Finally, control and rabbit myocardium specimens with experimentally induced anthracycline cardiomyopathy were analysed. Nearly 4000 myocardial proteins were quantified using µLC-MS/MS. In concordance with nLC-MS/MS, data evaluation revealed that severe anthracycline cardiotoxicity phenotype was associated with marked global protein dysregulation. Observed protein dysregulation consisted of profound remodelling of cardiomyocyte sarcomere as well as components of extracellular matrix. Pro-inflammatory proteins and multiple antioxidant proteins were markedly changed, whereas the levels of mitochondrial proteins were declined compared to control myocardium. In this study, µLC-MS/MS was confirmed as a versatile proteomic tool for comprehensive mapping of proteome changes related to anthracycline cardiotoxicity having a great potential to become full-fledged alternative to nLC.
HostingRepository	PRIDE
AnnounceDate	2025-04-30
AnnouncementXML	Submission_2025-04-30_06:11:40.909.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rudolf Kupcik
SpeciesList	scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986;
ModificationList	methylthiolated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-16 03:24:07	ID requested
⏵ 1	2025-04-30 06:11:45	announced

10.1021/acsomega.4c09377;

Kup, č, í, k R, Len, č, ov, á O, Mazurov, á Y, Š, t, ě, rba M, Vajrychov, á M, LC-MS/MS for Wide-Scale Proteomic Analysis of Anthracycline-Induced Cardiomyopathy. ACS Omega, 10(12):11980-11993(2025) [pubmed]

submitter keyword: cardiotoxicity, TMT, 2D-separation,microLC-MS/MS, daunorubicin

Marie Vajrychova
contact affiliation	Biomedical Research Centre, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic
contact email	marie.vajrychova@fnhk.cz
lab head
Rudolf Kupcik
contact affiliation	Biomedical Research Centre, University Hospital Hradec Kralove, Hradec Kralove
contact email	rudolf.kupcik@fnhk.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047014
     1. Label: PRIDE project
     2. Name: Methodological aspects of µLC-MS/MS for wide-scale proteomic analysis of anthracycline-induced cardiomyopathy