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PXD047014

PXD047014 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMethodological aspects of µLC-MS/MS for wide-scale proteomic analysis of anthracycline-induced cardiomyopathy
DescriptionThe effort to utilize microflow liquid chromatography for deep-scale proteomic analysis (µLC-MS/MS) is still growing. In this work, we utilized two-dimensional LC separation and peptide derivatization by tandem mass tag and assessed the capability of µLC-MS/MS to reveal protein changes associated with severe anthracycline-induced cardiomyopathy phenotype in comparison with nLC-MS/MS. Finally, control and rabbit myocardium specimens with experimentally induced anthracycline cardiomyopathy were analysed. Nearly 4000 myocardial proteins were quantified using µLC-MS/MS. In concordance with nLC-MS/MS, data evaluation revealed that severe anthracycline cardiotoxicity phenotype was associated with marked global protein dysregulation. Observed protein dysregulation consisted of profound remodelling of cardiomyocyte sarcomere as well as components of extracellular matrix. Pro-inflammatory proteins and multiple antioxidant proteins were markedly changed, whereas the levels of mitochondrial proteins were declined compared to control myocardium. In this study, µLC-MS/MS was confirmed as a versatile proteomic tool for comprehensive mapping of proteome changes related to anthracycline cardiotoxicity having a great potential to become full-fledged alternative to nLC.
HostingRepositoryPRIDE
AnnounceDate2025-04-30
AnnouncementXMLSubmission_2025-04-30_06:11:40.909.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRudolf Kupcik
SpeciesList scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986;
ModificationListmethylthiolated residue; acetylated residue; monohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-16 03:24:07ID requested
12025-04-30 06:11:45announced
Publication List
10.1021/acsomega.4c09377;
Kup, č, í, k R, Len, č, ov, á O, Mazurov, á Y, Š, t, ě, rba M, Vajrychov, á M, LC-MS/MS for Wide-Scale Proteomic Analysis of Anthracycline-Induced Cardiomyopathy. ACS Omega, 10(12):11980-11993(2025) [pubmed]
Keyword List
submitter keyword: cardiotoxicity, TMT, 2D-separation,microLC-MS/MS, daunorubicin
Contact List
Marie Vajrychova
contact affiliationBiomedical Research Centre, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic
contact emailmarie.vajrychova@fnhk.cz
lab head
Rudolf Kupcik
contact affiliationBiomedical Research Centre, University Hospital Hradec Kralove, Hradec Kralove
contact emailrudolf.kupcik@fnhk.cz
dataset submitter
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Dataset FTP location
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