PXD047014 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Methodological aspects of µLC-MS/MS for wide-scale proteomic analysis of anthracycline-induced cardiomyopathy |
Description | The effort to utilize microflow liquid chromatography for deep-scale proteomic analysis (µLC-MS/MS) is still growing. In this work, we utilized two-dimensional LC separation and peptide derivatization by tandem mass tag and assessed the capability of µLC-MS/MS to reveal protein changes associated with severe anthracycline-induced cardiomyopathy phenotype in comparison with nLC-MS/MS. Finally, control and rabbit myocardium specimens with experimentally induced anthracycline cardiomyopathy were analysed. Nearly 4000 myocardial proteins were quantified using µLC-MS/MS. In concordance with nLC-MS/MS, data evaluation revealed that severe anthracycline cardiotoxicity phenotype was associated with marked global protein dysregulation. Observed protein dysregulation consisted of profound remodelling of cardiomyocyte sarcomere as well as components of extracellular matrix. Pro-inflammatory proteins and multiple antioxidant proteins were markedly changed, whereas the levels of mitochondrial proteins were declined compared to control myocardium. In this study, µLC-MS/MS was confirmed as a versatile proteomic tool for comprehensive mapping of proteome changes related to anthracycline cardiotoxicity having a great potential to become full-fledged alternative to nLC. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-30 |
AnnouncementXML | Submission_2025-04-30_06:11:40.909.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rudolf Kupcik |
SpeciesList | scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986; |
ModificationList | methylthiolated residue; acetylated residue; monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-16 03:24:07 | ID requested | |
⏵ 1 | 2025-04-30 06:11:45 | announced | |
Publication List
10.1021/acsomega.4c09377; |
Kup, č, í, k R, Len, č, ov, á O, Mazurov, á Y, Š, t, ě, rba M, Vajrychov, á M, LC-MS/MS for Wide-Scale Proteomic Analysis of Anthracycline-Induced Cardiomyopathy. ACS Omega, 10(12):11980-11993(2025) [pubmed] |
Keyword List
submitter keyword: cardiotoxicity, TMT, 2D-separation,microLC-MS/MS, daunorubicin |
Contact List
Marie Vajrychova |
contact affiliation | Biomedical Research Centre, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic |
contact email | marie.vajrychova@fnhk.cz |
lab head | |
Rudolf Kupcik |
contact affiliation | Biomedical Research Centre, University Hospital Hradec Kralove, Hradec Kralove |
contact email | rudolf.kupcik@fnhk.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD047014 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047014
- Label: PRIDE project
- Name: Methodological aspects of µLC-MS/MS for wide-scale proteomic analysis of anthracycline-induced cardiomyopathy