PXD047005

PXD047005 is an original dataset announced via ProteomeXchange.

Title	In vitro phosphorylation of Dam1 complex and Bim1 by Mps1
Description	The Dam1 complex (Dam1c) of Saccharomyces cerevisiae is a major microtubule binding component of the yeast kinetochore. Phosphorylation of various kinetochore proteins by mitotic kinases such as Mps1 are known to modulate the function of these proteins and regulate protein-protein interaction. The Dam1 subunit of Dam1c was previously demonstrated to be phosphorylate by Mps1 (residues S13, S49, S217, S218, S221, S232. In this project we mapped additional phosphor sites on components of the Dam1 complex by mass spectrometry after in vitro phosphorylation as a first step to understand the physiological function of these phosphorylation events.
HostingRepository	PRIDE
AnnounceDate	2024-05-22
AnnouncementXML	Submission_2024-05-21_21:40:30.842.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Farnusch Kaschani
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-15 21:22:55	ID requested
⏵ 1	2024-05-21 21:40:31	announced

10.1016/J.CUB.2024.03.062;

submitter keyword: Mps1

phosphorylation,Dam1, mass spectrometry

Farnusch Kaschani
contact affiliation	Analytics Core Facility Essen (ACE), Chemische Biologie, Universität Duisburg-Essen, ZMB, Germany
contact email	farnusch.kaschani@uni-due.de
lab head
Farnusch Kaschani
contact affiliation	University Duisburg-Essen
contact email	farnusch.kaschani@uni-due.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD047005

PRIDE project URI

• PRIDE
  1. PXD047005
     1. Label: PRIDE project
     2. Name: In vitro phosphorylation of Dam1 complex and Bim1 by Mps1