PXD046997

PXD046997 is an original dataset announced via ProteomeXchange.

Title	Steap4 controls adipocyte thermogenesis and energy expenditure by modulating mitochondrial function
Description	Steap4, a highly expressed protein in adipose tissue, has been implicated in metabolic homeostasis. In this study, we generated adipocyte-specific Steap4-deficient mice and observed that Steap4 deficiency led to increased fat mass and severe insulin resistance in a high-fat diet model. Mass spectrometry analysis revealed two classes of Steap4 interactomes: mitochondrial proteins and proteins involved in spliceosome. RNA-seq analysis of white adipose tissue demonstrated that Steap4 deficiency altered RNA splicing patterns with enriched functions in mitochondria. While interactome and transcriptome data implicate a role of Steap4 in mitochondria, Steap4 deficiency indeed impaired mitochondrial respiratory chain complex activity resulting in mitochondrial dysfunction in white adipose tissue. Consistently, brown adipocyte-specific Steap4-deficient mice also showed impaired mitochondrial function, increased whitening of brown adipose tissue, reduced energy expenditure, and exacerbated insulin resistance under HFD conditions. Overall, our findings elucidate the critical role of Steap4 in regulating adipocyte thermogenesis and energy expenditure by modulating mitochondrial function.
HostingRepository	PRIDE
AnnounceDate	2025-02-03
AnnouncementXML	Submission_2025-02-03_09:55:14.708.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Belinda Willard
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-15 11:59:10	ID requested
⏵ 1	2025-02-03 09:55:15	announced

10.1016/J.ISCI.2025.111903;

submitter keyword: Adipose, STEAP4, IP-MS

Xiao Li, Ph.D
contact affiliation	1Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH, 44106
contact email	xiao.li9@case.edu
lab head
Belinda Willard
contact affiliation	Cleveland Clinic
contact email	willarb@ccf.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD046997

PRIDE project URI

• PRIDE
  1. PXD046997
     1. Label: PRIDE project
     2. Name: Steap4 controls adipocyte thermogenesis and energy expenditure by modulating mitochondrial function