PXD046983

PXD046983 is an original dataset announced via ProteomeXchange.

Title	Deep Quantitative Spectral Library of Normal and Myopic Mice Retinas: Proteomics Dataset from SWATH and DIA-NN
Description	The retina plays a crucial role in processing and decoding visual information, both in normal development and during myopia progression. Recent advancements have introduced a library-independent approach for data-independent acquisition (DIA) analyses. This study demonstrates deep proteome identification and quantification in individual mice retinas during myopia development, with an average of 6,263 ± 86 unique protein groups. We anticipate that this robust quantification and in-depth retinal-specific spectral library will contribute to a better understanding of the proteome complexity of retina. Furthermore, a comprehensive mice retinal-specific spectral library was generated, encompassing a total identification of 9,401 protein groups, 70,041 peptides, 95,339 precursors, and 761,868 transitions acquired using SWATH-MS acquisition on a ZenoTOF 7600 mass spectrometer. This dataset surpasses the spectral library generated through high-pH reversed-phase fractionation by data-dependent acquisition (DDA). The data is available via ProteomeXchange with the identifier PXD046983. It will also serve as an indispensable reference for investigations in myopia research and other retinal or neurological diseases.
HostingRepository	PRIDE
AnnounceDate	2024-07-19
AnnouncementXML	Submission_2024-07-19_01:59:27.004.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuen Lam
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	ZenoTOF 7600

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-15 03:40:03	ID requested
⏵ 1	2024-07-19 01:59:27	announced

Dataset with its publication pending

submitter keyword: Retina, DIA-NN,Myopia, Mice, SWATH-MS

Thomas Chuen
contact affiliation	School of Optometry, The Hong Kong Polytechnic University, Hong Kong
contact email	thomas.c.lam@polyu.edu.hk
lab head
Chuen Lam
contact affiliation	The Hong Kong Polytechnic University/School of optometry
contact email	cclam@polyu.edu.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD046983

PRIDE project URI

• PRIDE
  1. PXD046983
     1. Label: PRIDE project
     2. Name: Deep Quantitative Spectral Library of Normal and Myopic Mice Retinas: Proteomics Dataset from SWATH and DIA-NN