PXD046967

PXD046967 is an original dataset announced via ProteomeXchange.

Title	TMT analysis of detergent-soluble and insoluble fractions from HEK293T cells in normal conditions and upon heat shock
Description	HEK293T cells were cultured according to standard protocols and heat shocked at 42oC for 6 hours. Detergent-soluble and insoluble fractions from HEK293T cells were obtained following the procedures described before. Specifically, HEK293T cells were lysed in NP40 lysis buffer (Invitrogen #FNN0021) with protease inhibitors by gentle pipetting. The cell homogenate was centrifuged at 14000 rpm and the supernatant was retrieved (detergent-soluble fraction). The remaining pellet was washed twice in ice-cold NP40 lysis buffer and then resuspended in urea buffer (RIPA buffer with 8M urea). The samples were then centrifuged at 14000 rpm for 10 min to pellet any remaining cell debris. The resulting supernatant (detergent-insoluble fraction) was retrieved and analyzed as explained below.
HostingRepository	PRIDE
AnnounceDate	2025-08-01
AnnouncementXML	Submission_2025-08-01_12:18:34.541.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kaiwen Yu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-14 14:57:22	ID requested
⏵ 1	2025-08-01 12:18:34	announced

10.1016/J.JBC.2025.110517;

submitter keyword: proteome solubility

thermal stress

heat shock

HEK293 cells

Fabio Demontis
contact affiliation	Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA
contact email	Fabio.Demontis@STJUDE.ORG
lab head
Kaiwen Yu
contact affiliation	St Jude Children's Hospital
contact email	kaiwen.yu@stjude.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD046967

PRIDE project URI

• PRIDE
  1. PXD046967
     1. Label: PRIDE project
     2. Name: TMT analysis of detergent-soluble and insoluble fractions from HEK293T cells in normal conditions and upon heat shock