PXD046953

PXD046953 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Midnolin stimulates proteasome activity necessary for lymphopoiesis and B cell cancer growth
Description	We identified the essential gene encoding midnolin in a genetic screen in which multiple Midn alleles caused reductions in peripheral B cells and T cell-independent antibody responses. Causation was confirmed in mice with targeted deletion of 4 of 6 MIDN protein isoforms. MIDN augmented proteasome activity in lymphocytes but few other cell types. By cryo-electron microscopy we showed that MIDN binds directly to the 26S proteasome. MIDN-deficient B cells displayed aberrant activation of the IRE-1/XBP-1 pathway of the unfolded protein response. Partial or complete MIDN deficiency suppressed B lymphoproliferation in three models of B cell malignancies. Thus, MIDN is required for proteasome activity in support of lymphopoiesis and malignant B cell proliferation over a broad range of differentiation states. Targeting MIDN in B cell malignancies may avoid off-tumor toxicities caused by proteasome inhibition throughout the body.
HostingRepository	MassIVE
AnnounceDate	2024-04-11
AnnouncementXML	Submission_2024-04-11_07:14:00.669.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Moresco
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-14 06:56:45	ID requested
⏵ 1	2024-04-11 07:14:01	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Midnolin, proteasome, lymphopoiesis, B cell cancer

submitter keyword: Midnolin, proteasome, lymphopoiesis, B cell cancer

Contact List

Bruce Beutler
contact affiliation	UT Southwestern Medical Center
contact email	Bruce.Beutler@utsouthwestern.edu
lab head
James Moresco
contact affiliation	UT Southwestern
contact email	james.moresco@utsouthwestern.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093397/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093397/