PXD046936

PXD046936 is an original dataset announced via ProteomeXchange.

Title	Development and validation of an ELISA to measure REG3E in canine blood
Description	Regenerating islet-derived proteins (REG) are upregulated in people with sepsis, pancreatitis, and gastrointestinal diseases. One member of the REG family, namely REG3E, was recently identified in pancreatic tissue and plasma of dogs, with high expression in pancreatitis and sepsis. Our objective was to develop and validate an ELISA to measure REG3E concentrations in canine blood. An indirect sandwich ELISA was successfully developed using recombinant canine REG3E protein and polyclonal anti-canine REG3E antibodies raised in guinea pigs and rabbits. Antibody specificity was assessed using Western blot and mass spectrometric analysis of protein purified from canine plasma using Protein A Sepharose High Performance SpinTrap columns.
HostingRepository	PRIDE
AnnounceDate	2024-06-10
AnnouncementXML	Submission_2024-06-10_03:51:00.998.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfred Heller
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-14 00:33:20	ID requested
⏵ 1	2024-06-10 03:51:01	announced

10.1111/VCP.13352;

submitter keyword: Gastrointestinal,Biomarker, Sepsis, Canine, Pancreatitis, Canis lupus familiaris, Pancreas

Laureen Peters
contact affiliation	Clinical Diagnostic Laboratory, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
contact email	laureen.peters@unibe.ch
lab head
Manfred Heller
contact affiliation	Proteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland
contact email	pmscf@dbmr.unibe.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046936
     1. Label: PRIDE project
     2. Name: Development and validation of an ELISA to measure REG3E in canine blood