PXD046934

PXD046934 is an original dataset announced via ProteomeXchange.

Title	Activity-based proteomic profiling (ABPP) employing a serine hydrolase-directed probe to identify changes in activity profiles of lipid hydrolase in lung tumors
Description	Lung tumors, as well as normal tumor-adjacent (NTA) tissue of non-small cell lung cancer (NSCLC) patients, were collected and subjected to labeling with a serine hydrolase-directed activity-based probe (ABP) immediately after excision. Labeling with the serine hydrolase-directed ABP allows for the detection of active lipid hydrolases, which are the focus of our investigation. The labeled proteins (corresponding to active enzymes at time of labeling) were enriched using a biotin-containing linker that was covalently attached using strain-promoted alkyne-azide cycloaddition (click chemistry). Shotgun proteomics of the on-bead digested enriched proteins of ABP-treated and control samples enabled identification of enriched proteins as well as comparison of activity profiles between NTA and tumor samples of NSCLC patients.
HostingRepository	PRIDE
AnnounceDate	2023-11-27
AnnouncementXML	Submission_2023-11-27_01:42:54.225.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophie Honeder
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-ethylmaleimide derivatized cysteine
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-14 00:06:51	ID requested
⏵ 1	2023-11-27 01:42:54	announced

Dataset with its publication pending

submitter keyword: ABPP, lipid hydrolysis, serine hydrolase, NSCLC, lipid hydrolase,Lung tumor, activity-based proteomic profiling, lipase

Ruth Birner-Gruenberger
contact affiliation	Faculty of Technical Chemistry, Institute of Chemical Technologies and Analytics, Technische Universität Wien, Getreidemarkt 9/164, 1060 Vienna, Austria
contact email	ruth.birner-gruenberger@tuwien.ac.at
lab head
Sophie Honeder
contact affiliation	Diagnostic and Research Institute of Pathology, Medical University of Graz, Stiftingtalstraße 6, 8010 Graz, Austria
contact email	sophie.honeder@medunigraz.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD046934

PRIDE project URI

• PRIDE
  1. PXD046934
     1. Label: PRIDE project
     2. Name: Activity-based proteomic profiling (ABPP) employing a serine hydrolase-directed probe to identify changes in activity profiles of lipid hydrolase in lung tumors