PXD046918

PXD046918 is an original dataset announced via ProteomeXchange.

Title	Repeated Exposure Enhanced Toxicity of Clarithromycin on Microcystis aeruginosa versus Single Exposure through Photosynthesis, Oxidative Stress and Energy Metabolism shift
Description	Antibiotics are increasingly detected in aquatic environments, and their potential ecological risk is a concern. However, most antibiotic toxicity studies were performed with single exposure experiments. Here, we studied the effects and mechanisms of repeated clarithromycin (CLA) exposure on the algae Microcystis aeruginosa compared to the results of a single exposure. The 96-h effective concentration of CLA was 13.37 μg/L upon single exposure but was reduced to 6.90 μg/L upon repeated exposure. Single-exposure CLA inhibited algal photosynthesis by disrupting energy absorption, dissipation and trapping, reaction center activation, and electron transport, thereby inducing oxidative stress and ultrastructural damage. CLA also upregulated glycolysis, pyruvate metabolism, and the tricarboxylic acid cycle. Stronger inhibition of algal growth was observed under repeated exposure via effects on photosynthetic pigments, biosynthesis of reaction center subunits, and electron transport, inducing more substantial oxidative damage. Furthermore, repeated exposure reduced carbohydrate utilization by blocking the pentose phosphate pathway, which changed the characteristics of extracellular polymeric substances and eventually impaired algal self-defense. Risk quotients calculated from repeated exposure were higher than 1, indicating significant ecological risks. This study elucidated the severe influence of repeated antibiotic exposure on algae, providing new insights into antibiotic risk assessment.
HostingRepository	iProX
AnnounceDate	2023-11-13
AnnouncementXML	Submission_2024-05-20_20:13:38.017.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kailai Ye
SpeciesList	scientific name: Microcystis aeruginosa G-01; NCBI TaxID: 1009696;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-13 19:43:03	ID requested
1	2023-11-13 19:43:21	announced
⏵ 2	2024-05-20 20:13:38	announced	2024-05-21: Update information.

Mao Y, Ye K, Yang S, Salam M, Yu W, He Q, He R, Li H, Versus Single Exposure through Photosynthesis, Oxidative Stress, and Energy Metabolism Shift. Environ Sci Technol, 58(9):4070-4082(2024) [pubmed]

submitter keyword: Microcystis aeruginosa, Clarithromycin, Repeated exposure, Metabolomics, Ecological risk

Jiang Hu
contact affiliation	Key Laboratory of Hydraulic and Waterway Engineering, Ministry of Education, Chongqing Jiaotong University
contact email	378922494@qq.com
lab head
Kailai Ye
contact affiliation	Key Laboratory of Hydraulic and Waterway Engineering, Ministry of Education, Chongqing Jiaotong University, Chongqing 400074, China
contact email	1545499257@qq.com
dataset submitter

iProX dataset URI