PXD046912

PXD046912 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of non-small cell lung cancer cell lines lacking ATGL (ATGL-KO) or MGL (MGL-KO) or expressing both (Control, NTC)
Description	Adipose Triglyceride Lipase (ATGL) and Monoglyceride Lipase (MGL) are two enzymes that contribute to intracellular neutral lipolysis by breaking down triglycerides stored within lipid droplets. Recently, lipid droplet accumulation has been described as a novel hallmark of cancer. While lipid metabolism has been investigated in cancer in recent decades, the role of lipid hydrolysis and its enzymes have not been in the focus of cancer research. We and others have found that lipid hydrolysis enzymes might play an important role in the development and progression of lung cancer. To this end, we chose four different non-small cell lung cancer cell lines and employed CRISPR-Cas9 gene editing to knock out either ATGL (ATGL-KO) or MGL (MGL-KO), and a non-targeting control (NTC) was employed to generate a control cell line within each parental cell type. We then performed label free quantitative proteomics to identify differences between the generated cell lines and confirmed ATGL-KO in ATGL-KO cell lines as well as MGL-KO in MGL-KO cell lines. Furthermore, dihydroorotate dehydrogenase (DHODH), an enzyme that is important in some cancer, was upregulated in some, but not all, of the NSCLC cancer cell lines lacking either one of the two lipases.
HostingRepository	PRIDE
AnnounceDate	2023-11-27
AnnouncementXML	Submission_2023-11-27_03:10:14.186.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophie Honeder
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-13 14:44:09	ID requested
⏵ 1	2023-11-27 03:10:14	announced

Dataset with its publication pending

submitter keyword: H1299, lipid hydrolysis, lipolysis,NSCLC, A549, ATGL, H358, H441, MGL, lipase

Ruth Birner-Gruenberger
contact affiliation	Faculty of Technical Chemistry, Institute of Chemical Technologies and Analytics, Technische Universität Wien, Getreidemarkt 9/164, 1060 Vienna, Austria
contact email	ruth.birner-gruenberger@tuwien.ac.at
lab head
Sophie Honeder
contact affiliation	Diagnostic and Research Institute of Pathology, Medical University of Graz, Stiftingtalstraße 6, 8010 Graz, Austria
contact email	sophie.honeder@medunigraz.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD046912

PRIDE project URI

• PRIDE
  1. PXD046912
     1. Label: PRIDE project
     2. Name: Proteomics analysis of non-small cell lung cancer cell lines lacking ATGL (ATGL-KO) or MGL (MGL-KO) or expressing both (Control, NTC)