PXD046906

PXD046906 is an original dataset announced via ProteomeXchange.

Title	Trypanosoma cruzi interaction with host tissues modulate the composition of large extracellular vesicles
Description	Trypanosoma cruzi is the protozoan that causes Chagas disease, an endemic parasitosis in Latin America that has spread around the globe. Recently, a series of studies indicate that the gastrointestinal tract represents an important reservoir for T. cruzi in the chronic phase. It is also known that, during contact between T. cruzi and host cells, there is a release of extracellular vesicles (EVs) that modulates the immune system and enhances the infection, but the dynamics of secretion of host and parasite molecules through these EVs is not understood. In this study, we used two cell lines to simulate the environments found by the parasite in the host: C2C12 cell (myoblast) and Caco-2 cell (intestinal epithelium). We isolated large EVs (LEVs) from the interaction of T. cruzi culture-derived trypomastigotes (TCTs) belonging to two distinct strains (CL Brener, DTU Tc VI and Dm28c DTU Tc I) in contact with C2C12 and Caco-2 cells to 2 hours and after 24 hours of infection. The interaction of the parasite with the host cell induces a switch in the functionality of proteins carried by LEVs and a varied tissue answer. Protein-protein interaction analysis indicates that LEVs carry key proteins for host-pathogen interaction that could participate in the pathogenesis of Chagas Disease.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:39:40.515.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rafael de Almeida
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Trypanosoma cruzi Dm28c; NCBI TaxID: 1416333; scientific name: Trypanosoma cruzi strain CL Brener; NCBI TaxID: 353153;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-13 07:20:43	ID requested
1	2024-05-17 10:05:18	announced
⏵ 2	2024-10-22 06:39:40	announced	2024-10-22: Updated project metadata.

10.1038/S41598-024-55302-3;

submitter keyword: mass spectrometry, tropism, microvesicles, extracellular vesicles,Trypanosoma cruzi

Marcel Ivan
contact affiliation	EVAHPI Research Group, Laboratório de Biologia Celular, Instituto Carlos Chagas, Fundação Oswaldo Cruz
contact email	marcel.ivan.ramirez@gmail.com
lab head
Rafael de Almeida
contact affiliation	Fiocruz
contact email	alosteria@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD046906

PRIDE project URI

• PRIDE
  1. PXD046906
     1. Label: PRIDE project
     2. Name: Trypanosoma cruzi interaction with host tissues modulate the composition of large extracellular vesicles