PXD046899

PXD046899 is an original dataset announced via ProteomeXchange.

Title	DiGly analysis of autoubiquitinated UBR4 (residues 4730-5183)
Description	Human recombinant UBR4 protein, consisting of residues 4730-5183, undergoes in vitro autoubiquitination when incubated with the E2 conjugating enzyme UBE2A and the other reaction components. The autoubiquitinated protein was analysed by mass spectrometry to identify modification sites and the ubiquitin linkage types present. This was achieved by searching for diGly as a variable modification on Ser, Thr, Tyr, Cys and Lys residues. A single autoubiquitination site at K4814 was detected. However, mutation of this residue impaired autoubiquitination, indicating the presence of additional sites. The ubiquitin linkages identified were Lys33, Lys48, Lys63, Lys11 and Lys6, suggesting that UBR4 assembles these ubiquitin linkages on its substrates.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:14:25.709.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frederic Lamoliatte
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-13 05:24:21	ID requested
1	2023-11-15 00:52:59	announced
⏵ 2	2024-10-22 06:14:26	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: autoubiquitinated,UBR4

Satpal Virdee
contact affiliation	Medical Research Council Protein Phosphorylation and Ubiquitylation Unit School of Life Sciences, University of Dundee, Dundee, Scotland
contact email	s.s.virdee@dundee.ac.uk
lab head
Frederic Lamoliatte
contact affiliation	University of Dundee
contact email	flamoliatte001@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD046899

PRIDE project URI

• PRIDE
  1. PXD046899
     1. Label: PRIDE project
     2. Name: DiGly analysis of autoubiquitinated UBR4 (residues 4730-5183)