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PXD046899

PXD046899 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDiGly analysis of autoubiquitinated UBR4 (residues 4730-5183)
DescriptionHuman recombinant UBR4 protein, consisting of residues 4730-5183, undergoes in vitro autoubiquitination when incubated with the E2 conjugating enzyme UBE2A and the other reaction components. The autoubiquitinated protein was analysed by mass spectrometry to identify modification sites and the ubiquitin linkage types present. This was achieved by searching for diGly as a variable modification on Ser, Thr, Tyr, Cys and Lys residues. A single autoubiquitination site at K4814 was detected. However, mutation of this residue impaired autoubiquitination, indicating the presence of additional sites. The ubiquitin linkages identified were Lys33, Lys48, Lys63, Lys11 and Lys6, suggesting that UBR4 assembles these ubiquitin linkages on its substrates.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:14:25.709.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrederic Lamoliatte
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitinylated lysine
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-13 05:24:21ID requested
12023-11-15 00:52:59announced
22024-10-22 06:14:26announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: autoubiquitinated,UBR4
Contact List
Satpal Virdee
contact affiliationMedical Research Council Protein Phosphorylation and Ubiquitylation Unit School of Life Sciences, University of Dundee, Dundee, Scotland
contact emails.s.virdee@dundee.ac.uk
lab head
Frederic Lamoliatte
contact affiliationUniversity of Dundee
contact emailflamoliatte001@dundee.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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