PXD046899 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | DiGly analysis of autoubiquitinated UBR4 (residues 4730-5183) |
Description | Human recombinant UBR4 protein, consisting of residues 4730-5183, undergoes in vitro autoubiquitination when incubated with the E2 conjugating enzyme UBE2A and the other reaction components. The autoubiquitinated protein was analysed by mass spectrometry to identify modification sites and the ubiquitin linkage types present. This was achieved by searching for diGly as a variable modification on Ser, Thr, Tyr, Cys and Lys residues. A single autoubiquitination site at K4814 was detected. However, mutation of this residue impaired autoubiquitination, indicating the presence of additional sites. The ubiquitin linkages identified were Lys33, Lys48, Lys63, Lys11 and Lys6, suggesting that UBR4 assembles these ubiquitin linkages on its substrates. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:14:25.709.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Frederic Lamoliatte |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | ubiquitinylated lysine |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-13 05:24:21 | ID requested | |
1 | 2023-11-15 00:52:59 | announced | |
⏵ 2 | 2024-10-22 06:14:26 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: autoubiquitinated,UBR4 |
Contact List
Satpal Virdee |
contact affiliation | Medical Research Council Protein Phosphorylation and Ubiquitylation Unit School of Life Sciences, University of Dundee, Dundee, Scotland |
contact email | s.s.virdee@dundee.ac.uk |
lab head | |
Frederic Lamoliatte |
contact affiliation | University of Dundee |
contact email | flamoliatte001@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046899
- Label: PRIDE project
- Name: DiGly analysis of autoubiquitinated UBR4 (residues 4730-5183)