⮝ Full datasets listing
PXD046896
PXD046896 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Leiomodin 1 promotes myogenic differentiation by modulating Sirtuin 1 localization |
| Description | During myogenesis, the cell architecture and thereby the proteome undergoes extensive remodelling to enable muscle development and regeneration, a process which is only partially characterised and understood and displays impairments in age and disease. By analysing the protein expression dynamics during myogenic differentiation using mass spectrometry we identified the actin nucleator protein leiomodin 1 (LMOD1) among a subset of proteins that increase abundance in early phases of myogenesis. Knockdown of Lmod1 in primary myoblasts severely affected the terminal differentiation and fusion of myotubes. We show that LMOD1 interacts with the deacetylase sirtuin 1 (SIRT1) and that depletion or pharmacological inhibition of SIRT1 partially rescues the effects of Lmod1 knockdown. Both LMOD1 and SIRT1 re-distribute from the nucleus to cytoplasm during myogenic differentiation and their localization correlates especially at the onset of myogenic differentiation. We show that overexpression of LMOD1 is sufficient to alter SIRT1 localization, de-repress SIRT1 target genes, promote accelerated myotube formation and improve myoblast fusion. Our work identifies a novel regulator of myogenesis that might be targeted to improve muscle regeneration in aging and disease. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-06-23 |
| AnnouncementXML | Submission_2026-06-23_12:08:20.039.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Leibniz FLI Proteomics |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Carbamyl; Carbamidomethyl |
| Instrument | Q Exactive HF; Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-11-13 04:48:07 | ID requested | |
| ⏵ 1 | 2026-06-23 12:08:20 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: muscle stem cells, MuSCs, cytoskeleton dynamics, myogenesis, Lmod1, Sirt1, mass spectrometry, differentiation |
Contact List
| Alessandro Ori | |
|---|---|
| contact affiliation | Leibniz Institute on Aging Fritz Lipmann Institute (FLI) |
| contact email | alessandro.ori@leibniz-fli.de |
| lab head | |
| Leibniz FLI Proteomics | |
| contact affiliation | Leibniz FLI |
| contact email | proteomics@leibniz-fli.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v06/MSV000093379/ |
