PXD046895

PXD046895 is an original dataset announced via ProteomeXchange.

Title	Hsp70 and NAC chaperone systems are in close proximity on the ribosome
Description	To understand the orientation of Hsp70 Ssb on the ribosome in living Saccharomyces cerevisiae cells we incorporated the noncanonical, photoactivatable amino acid p-benzoyl-L-phenylalanine (Bpa) in place of specific individual endogenous amino acids in Ssb1. To identify the proteins to which Ssb1Bpa crosslinked we performed mass spectrometry. The results revealed that Bpa incorporated at the tip of the “lid” subdomain cross linked to the nascent chain associated complex (NAC), while Bpa incorporated into further down the lid crosslinked to ribosomal protein uL29.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:19:38.702.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046895
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marcin Pitek
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-13 03:26:40	ID requested
1	2024-01-08 11:29:59	announced
⏵ 2	2024-10-22 06:19:39	announced	2024-10-22: Updated project metadata.

10.6019/PXD046895;

10.1093/NAR/GKAE005;

submitter keyword: NAC, Ssb1, ribosome, in vivo crosslinking, Hsp70

Elizabeth A. Craig
contact affiliation	Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53726, United States of America
contact email	ecraig@wisc.edu
lab head
Marcin Pitek
contact affiliation	Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk
contact email	marcin.pitek@phdstud.ug.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD046895

PRIDE project URI

• PRIDE
  1. PXD046895
     1. Label: PRIDE project
     2. Name: Hsp70 and NAC chaperone systems are in close proximity on the ribosome