PXD046895 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Hsp70 and NAC chaperone systems are in close proximity on the ribosome |
Description | To understand the orientation of Hsp70 Ssb on the ribosome in living Saccharomyces cerevisiae cells we incorporated the noncanonical, photoactivatable amino acid p-benzoyl-L-phenylalanine (Bpa) in place of specific individual endogenous amino acids in Ssb1. To identify the proteins to which Ssb1Bpa crosslinked we performed mass spectrometry. The results revealed that Bpa incorporated at the tip of the “lid” subdomain cross linked to the nascent chain associated complex (NAC), while Bpa incorporated into further down the lid crosslinked to ribosomal protein uL29. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:19:38.702.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD046895 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Marcin Pitek |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-13 03:26:40 | ID requested | |
1 | 2024-01-08 11:29:59 | announced | |
⏵ 2 | 2024-10-22 06:19:39 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: NAC, Ssb1, ribosome, in vivo crosslinking, Hsp70 |
Contact List
Elizabeth A. Craig |
contact affiliation | Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53726, United States of America |
contact email | ecraig@wisc.edu |
lab head | |
Marcin Pitek |
contact affiliation | Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk |
contact email | marcin.pitek@phdstud.ug.edu.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD046895 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046895
- Label: PRIDE project
- Name: Hsp70 and NAC chaperone systems are in close proximity on the ribosome