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PXD046895

PXD046895 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHsp70 and NAC chaperone systems are in close proximity on the ribosome
DescriptionTo understand the orientation of Hsp70 Ssb on the ribosome in living Saccharomyces cerevisiae cells we incorporated the noncanonical, photoactivatable amino acid p-benzoyl-L-phenylalanine (Bpa) in place of specific individual endogenous amino acids in Ssb1. To identify the proteins to which Ssb1Bpa crosslinked we performed mass spectrometry. The results revealed that Bpa incorporated at the tip of the “lid” subdomain cross linked to the nascent chain associated complex (NAC), while Bpa incorporated into further down the lid crosslinked to ribosomal protein uL29.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:19:38.702.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD046895
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMarcin Pitek
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-13 03:26:40ID requested
12024-01-08 11:29:59announced
22024-10-22 06:19:39announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD046895;
10.1093/NAR/GKAE005;
Keyword List
submitter keyword: NAC, Ssb1, ribosome, in vivo crosslinking, Hsp70
Contact List
Elizabeth A. Craig
contact affiliationDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53726, United States of America
contact emailecraig@wisc.edu
lab head
Marcin Pitek
contact affiliationIntercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk
contact emailmarcin.pitek@phdstud.ug.edu.pl
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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