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PXD046892

PXD046892 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn inhibitory segment within G-patch activators tunes Prp43-ATPase activity during ribosome assembly
DescriptionMechanisms by which G-patch activators tune the processive multi-tasking ATP-dependent RNA helicase Prp43 to remodel diverse RNA:protein complexes remain elusive. Here, a comparative study between a new activator, Tma23, and a characterized activator, Pxr1, uncovered segments that organize Prp43 function during ribosome assembly. In addition to the activating G-patch, we discovered an inhibitory segment within Tma23 and Pxr1, I-patch, that restrains Prp43-ATPase activity. Cryo-electron microscopy and hydrogen-deuterium exchange mass spectrometry show how I-patch binds to the catalytic RecA-like domains to allosterically inhibit Prp43-ATPase activity. Tma23 and Pxr1 also contain a dimerization segment which organizes Prp43 into higher-order complexes. We posit that Prp43 function at discrete locations on pre-ribosomal RNA are coordinated through toggling interactions with G-patch and I-patch motifs. This could guarantee measured and timely Prp43 activation, enabling precise control over multiple RNA remodeling events occurring concurrently during ribosome formation.
HostingRepositoryPRIDE
AnnounceDate2024-10-09
AnnouncementXMLSubmission_2024-10-09_08:31:12.470.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD046892
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterOscar Vadas
SpeciesList scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-13 02:16:44ID requested
12024-10-09 08:31:12announced
Publication List
10.6019/PXD046892;
Keyword List
submitter keyword: Ribosome assembly, Prp43, Cryo-EM, HDX-MS, RNA helicase
Contact List
Oscar Vadas
contact affiliationUniversity of Geneva, Faculty of Medicine, Protein Platform, Geneva, Switzerland
contact emailoscar.vadas@unige.ch
lab head
Oscar Vadas
contact affiliationUniversity of Geneva, Faculty of Medicine
contact emailoscar.vadas@unige.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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