PXD046892

PXD046892 is an original dataset announced via ProteomeXchange.

Title	An inhibitory segment within G-patch activators tunes Prp43-ATPase activity during ribosome assembly
Description	Mechanisms by which G-patch activators tune the processive multi-tasking ATP-dependent RNA helicase Prp43 to remodel diverse RNA:protein complexes remain elusive. Here, a comparative study between a new activator, Tma23, and a characterized activator, Pxr1, uncovered segments that organize Prp43 function during ribosome assembly. In addition to the activating G-patch, we discovered an inhibitory segment within Tma23 and Pxr1, I-patch, that restrains Prp43-ATPase activity. Cryo-electron microscopy and hydrogen-deuterium exchange mass spectrometry show how I-patch binds to the catalytic RecA-like domains to allosterically inhibit Prp43-ATPase activity. Tma23 and Pxr1 also contain a dimerization segment which organizes Prp43 into higher-order complexes. We posit that Prp43 function at discrete locations on pre-ribosomal RNA are coordinated through toggling interactions with G-patch and I-patch motifs. This could guarantee measured and timely Prp43 activation, enabling precise control over multiple RNA remodeling events occurring concurrently during ribosome formation.
HostingRepository	PRIDE
AnnounceDate	2024-10-09
AnnouncementXML	Submission_2024-10-09_08:31:12.470.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046892
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Oscar Vadas
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-13 02:16:44	ID requested
⏵ 1	2024-10-09 08:31:12	announced

10.6019/PXD046892;

submitter keyword: Ribosome assembly, Prp43, Cryo-EM, HDX-MS, RNA helicase

Oscar Vadas
contact affiliation	University of Geneva, Faculty of Medicine, Protein Platform, Geneva, Switzerland
contact email	oscar.vadas@unige.ch
lab head
Oscar Vadas
contact affiliation	University of Geneva, Faculty of Medicine
contact email	oscar.vadas@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD046892

PRIDE project URI

• PRIDE
  1. PXD046892
     1. Label: PRIDE project
     2. Name: An inhibitory segment within G-patch activators tunes Prp43-ATPase activity during ribosome assembly