PXD046891

PXD046891 is an original dataset announced via ProteomeXchange.

Title	Loss of p14 reduces immunogenicity in melanoma by affecting the peptide surface density via non-canonical Wnt signaling
Description	CDKN2A and its two gene products p14 and p16 are one of the most frequently negatively altered gene loci (either by loss or mutation) in advanced melanoma accompanied by decreased T cell infiltration in melanoma. In this study, we shed light on the connection of the reduction of the CDKN2A gene product p14 and its impact on melanoma immunogenicity. Knockdown of p14 in melanoma cell lines diminishes the recognition and killing by melanoma differentiation antigen (MDA) specific T cells. Resistance was caused by a reduction of surface density of presented differentiation surface antigens. While antigen presentation via HLA-I molecules was enhanced upon p14 downregulation in general, absolute and relative expression of cognate peptides was decreased. Limiting Wnt5a signaling reverted this phenotype showing involvement of non-canonical Wnt signaling. Taken together, our data indicate a new mechanism limiting MDA specific T cell responses by decreasing both absolute and relative MDA-peptide presentation in melanoma.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_12:02:24.388.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Melissa Bernhardt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C; 6x(13)C; deuterium tetrasubstituted residue; 3x(2)H labeled L-leucine; 6x(13)C labeled L-leucine; 6x(13)C labeled L-arginine
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-13 01:43:29	ID requested
⏵ 1	2025-05-06 12:02:25	announced

Wohlfarth J, Kosnopfel C, Faber D, Berthold M, Siedel C, Bernhardt M, Schlosser A, Aprati T, Liu D, Schrama D, Houben R, Schadendorf D, Goebeler M, Meierjohann S, Schilling B, Loss of p14 diminishes immunogenicity in melanoma via non-canonical Wnt signaling by reducing the peptide surface density. Mol Oncol, 18(10):2449-2470(2024) [pubmed]

10.1002/1878-0261.13660;

submitter keyword: HLA,human immunopeptidome, differnetiation antigen, melanoma

Andreas Schlosser
contact affiliation	Lab head of mass spectrometry
contact email	andreas.schlosser@uni-wuerzburg.de
lab head
Melissa Bernhardt
contact affiliation	mass spectrometry
contact email	melissa.bernhardt@uni-wuerzburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD046891

PRIDE project URI

• PRIDE
  1. PXD046891
     1. Label: PRIDE project
     2. Name: Loss of p14 reduces immunogenicity in melanoma by affecting the peptide surface density via non-canonical Wnt signaling