PXD046884

PXD046884 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of protein abundance after dEALK1 or dEALK1Me treatment in H3122 cells non-small cell lung cancer cells
Description	In this study, we designed and synthesized a PROTAC named dEALK1 to degrade EML4-ALK protein, and also dEALK1Me that cannot exert degradation function served as a negative control. Mass spectrometry-based tandem mass tag-labeled quantitative proteomics was performed to evaluate the potential off-target effect of dEALK1 in H3122 cells treated with dEALK1 or dEALK1Me for six hours. The results showed that significant depletion of EML4-ALK proteins was only identified in cells treated with dEALK1, but not dEALK1Me, indicating that dEALK1 exerts a highly selective effect on stability of EML4-ALK protein.
HostingRepository	iProX
AnnounceDate	2023-11-12
AnnouncementXML	Submission_2024-02-26_20:07:01.401.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-12 19:23:28	ID requested
1	2024-01-10 22:18:21	announced
⏵ 2	2024-02-26 20:07:02	announced	2024-02-27: Update information.

Li X, Wang Z, Chen C, Yang F, Liu P, Fang S, Wang B, Chen S, Li X, -rearranged lung cancer with ceritinib resistance. iScience, 27(2):109015(2024) [pubmed]

submitter keyword: PROTAC, non-small cell lung cancer, EML4-ALK

Xinjian Li
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences
contact email	lixinjian@ibp.ac.cn
lab head
Xin Li
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences
contact email	18333159079@163.com
dataset submitter

iProX dataset URI