PXD046871

PXD046871 is an original dataset announced via ProteomeXchange.

Title	Temporal Quantitative Phosphoproteomic Profiling of SH-SY5Y and IMR-32 Neuroblastoma Cells During All-trans Retinoic Acid-Induced Neuronal Differentiation
Description	The human neuroblastoma cell lines SH-SY5Y and IMR-32 can be differentiated into neuron-like phenotypes through treatment with all-trans retinoic acid (ATRA). After differentiation, these cell lines are extensively utilized as in vitro models to study various aspects of neuronal cell biology. However, temporal and quantitative profiling of the proteome and phosphoproteome of SH-SY5Y and IMR-32 cells throughout ATRA-induced differentiation has been limited. Here, we performed relative quantification of the phosphoproteomes of SH-SY5Y and IMR-32 cells at multiple time points during ATRA-induced differentiation. The data presented serve as a valuable resource for investigating temporal protein and phosphoprotein abundance changes in SH-SY5Y and IMR-32 cells during ATRA-induced differentiation.
HostingRepository	PRIDE
AnnounceDate	2024-01-24
AnnouncementXML	Submission_2024-01-23_22:46:53.391.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas.C.N. LEUNG
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; phosphorylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-12 03:28:23	ID requested
⏵ 1	2024-01-23 22:46:54	announced

Leung TCN, Lu SN, Chu CN, Lee J, Liu X, Ngai SM, -Retinoic Acid-Induced Neuronal Differentiation. Int J Mol Sci, 25(2):(2024) [pubmed]

10.3390/ijms25021047;

submitter keyword: Phosphoproteomics,SH-SY5Y, IMR-32, Neuronal Differentiation, TMT

Thomas C.N. LEUNG
contact affiliation	School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China
contact email	cnleung0509@gmail.com
lab head
Thomas.C.N. LEUNG
contact affiliation	School of Life Sciences, State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong
contact email	cnleung0509@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046871
     1. Label: PRIDE project
     2. Name: Temporal Quantitative Phosphoproteomic Profiling of SH-SY5Y and IMR-32 Neuroblastoma Cells During All-trans Retinoic Acid-Induced Neuronal Differentiation