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PXD046863

PXD046863 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBeyond Myosin Heavy Chains in Human Skeletal Muscle Heterogeneity
DescriptionSkeletal muscle is an inherently heterogenous tissue comprised primarily of myofibers, which are historically classified into three distinct fiber types in humans: one “slow” (type 1) and two “fast” (type 2A and type 2X), delineated by the expression of myosin heavy chain isoforms (MYHs). However, whether discrete fiber types exist or whether fiber heterogeneity reflects a continuum remains unclear. Furthermore, whether MYHs are the main classifiers of skeletal muscle fibers has not been examined in an unbiased manner. Through the development and application of novel transcriptomic and proteomic workflows, applied to 1050 and 1038 single muscle fibers from human vastus lateralis, respectively, we show that MYHs are not the principal drivers of skeletal muscle fiber heterogeneity. Instead, ribosomal heterogeneity drives the majority of variance between skeletal muscle fibers in a continual fashion, independent of slow/fast fiber type. Furthermore, whilst slow and fast fiber clusters can be identified, described by their contractile and metabolic profiles, our data challenge the concept that type 2X are phenotypically distinct from other fast fibers at an omics level. Moreover, MYH-based classifications do not adequately describe the phenotype of skeletal muscle fibers in one of the most common genetic muscle diseases, nemaline myopathy. Our data question the currently accepted model of multiple distinct fiber types based on the expression of MYHs in humans and identifies ribosomal heterogeneity as a major driver of skeletal muscle fiber heterogeneity, opening a new field of research within skeletal muscle physiology.
HostingRepositoryPRIDE
AnnounceDate2025-05-09
AnnouncementXMLSubmission_2025-05-09_00:07:51.190.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRoger Moreno Justicia
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF SCP
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-11 05:56:27ID requested
12025-05-09 00:07:52announced
Publication List
10.1038/s41467-025-56896-6;
Moreno-Justicia R, Van der Stede T, Stocks B, Laitila J, Seaborne RA, Van de Loock A, Lievens E, Samodova D, Mar, í, n-Arraiza L, Dmytriyeva O, Browaeys R, Van Vossel K, Moesgaard L, Yigit N, Anckaert J, Weyns A, Van Thienen R, Sahl RE, Zanoteli E, Lawlor MW, Wierer M, Mestdagh P, Vandesompele J, Ochala J, Hostrup M, Derave W, Deshmukh AS, Human skeletal muscle fiber heterogeneity beyond myosin heavy chains. Nat Commun, 16(1):1764(2025) [pubmed]
Keyword List
submitter keyword: Single muscle fiber
cell heterogeneity
myopathy
fiber type
Contact List
Atul Shahaji
contact affiliationNovo Nordisk Foundation Center for Basic Metabolic Research
contact emailatul.deshmukh@sund.ku.dk
lab head
Roger Moreno Justicia
contact affiliationNovo Nordisk Foundation Center for Basic Metabolic Research
contact emailroger.moreno.justicia@sund.ku.dk
dataset submitter
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Dataset FTP location
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PRIDE project URI
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