PXD046860

PXD046860 is an original dataset announced via ProteomeXchange.

Title	Integrated multi-omics and genetic analyses reveal molecular determinants underlying Arabidopsis snap33 mutant phenotype
Description	The secretory pathway is essential for plant immunity, delivering diverse antimicrobial molecules into the extracellular space. Arabidopsis thaliana soluble N-ethylmaleimide-sensitive-factor attachment protein receptor SNAP33 is a key actor of this process. The snap33 mutant displays dwarfism and necrotic lesions, however the molecular determinants of its macroscopic phenotypes remain elusive. Here, we isolated several new snap33 mutants that exhibited constitutive cell death and H2O2 accumulation, further defining snap33 as an autoimmune mutant. We then carried out quantitative transcriptomic and proteomic analyses showing that numerous defense transcripts and proteins were up-regulated in snap33 mutant, among which genes/proteins involved in defense hormone, pattern-triggered immunity and nucleotide-binding domain leucine-rich-repeat receptor signaling. qRT-PCR analyses and hormone dosages supported these results. Furthermore, genetic analyses elucidated the diverse contributions of the main defense hormones and of some nucleotide-binding domain leucine-rich-repeat receptor signaling actors in the establishment of snap33 phenotype, emphasizing the preponderant role of salicylic acid over other defense phytohormones. Moreover, the accumulation of pattern-triggered immunity and nucleotide-binding domain leucine-rich-repeat receptor signaling proteins in snap33 was confirmed by immunoblotting analyses and further shown to be salicylic acid-dependent. Collectively, this study unveiled molecular determinants underlying Arabidopsis snap33 mutant phenotype and brought new insights into autoimmunity signaling.
HostingRepository	PRIDE
AnnounceDate	2024-02-01
AnnouncementXML	Submission_2024-01-31_23:10:48.210.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Naganand Rayapuram
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-11 00:00:58	ID requested
⏵ 1	2024-01-31 23:10:49	announced

10.1111/tpj.16647;

Henchiri H, Rayapuram N, Alhoraibi HM, Ca, ï, us J, Paysant-Le Roux C, Citerne S, Hirt H, Colcombet J, Sturbois B, Bigeard J, Integrated multi-omics and genetic analyses reveal molecular determinants underlying Arabidopsis snap33 mutant phenotype. Plant J, ():(2024) [pubmed]

submitter keyword: Arabidopsis, ETI, salicylic acid, MAPK, SNAP33, PTI, autoimmunity, NLR

Naganand Rayapuram
contact affiliation	King Abdullah University of Science and Technology, Thuwal, Saudi Arabia
contact email	naganand.rayapuram@kaust.edu.sa
lab head
Naganand Rayapuram
contact affiliation	KAUST
contact email	naganand.rayapuram@kaust.edu.sa
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD046860

PRIDE project URI

• PRIDE
  1. PXD046860
     1. Label: PRIDE project
     2. Name: Integrated multi-omics and genetic analyses reveal molecular determinants underlying Arabidopsis snap33 mutant phenotype