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PXD046846

PXD046846 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCD99 modulates the proteomic landscape of Ewing sarcoma cells and related-extracellular vesicles.
DescriptionEwing sarcoma (EWS) is an aggressive pediatric bone tumor characterized by unmet clinical needs and an incompletely understood epigenetic heterogeneity. Here we considered CD99, a major surface molecule hallmark of EWS malignancy. Fluctuations of CD99 expression strongly impair cell dissemination, differentiation, and death. CD99 is also loaded within extracellular vesicles (EVs) and the delivery of CD99 positive or CD99 negative EVs dynamically exerts oncogenic or oncosuppressive functions to recipient cells, respectively. We undertook mass spectrometry and functional annotation analysis to investigate the consequences of CD99 silencing on the proteomic landscape of EWS cells and related EVs. Our data demonstrate that i. the decrease of CD99 leads to major changes in the proteomic profile of EWS cells and EVs; ii. intracellular and extracellular compartments display two distinct signatures of differentially expressed proteins; iii. proteomic changes converge to the modulation of cell migration and immune-modulation biological processes; iv. CD99-silenced cells and related EVs are characterized by a migration-suppressive, pro-immunostimulatory proteomic profile. Overall, our data provide a novel source of CD99-associated protein biomarkers to be considered for further validation as mediators of EWS malignancy and as EWS disease liquid biopsy markers.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:41:26.871.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarcello Manfredi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListcarbamoylated residue; monohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-10 06:14:21ID requested
12024-05-22 11:44:02announced
22024-10-22 06:41:33announced2024-10-22: Updated project metadata.
Publication List
De Feo A, Manfredi M, Mancarella C, Maqueda JJ, De Giorgis V, Pignochino Y, Sciandra M, Cristalli C, Donadelli M, Scotlandi K, CD99 Modulates the Proteomic Landscape of Ewing Sarcoma Cells and Related Extracellular Vesicles. Int J Mol Sci, 25(3):(2024) [pubmed]
10.3390/ijms25031588;
Keyword List
submitter keyword: Ewing sarcoma
heterogeneity
proteomic
mass spectrometry
biomarkers
extracellular vesicles.
Contact List
Marcello Manfredi
contact affiliationMarcello Manfredi PhD Biological Mass Spectrometry Lab www.biomass-spec.com Department of Translational Medicine (DiMeT) https://www.agingproject.uniupo.it/ Center for Translational Research on Autoimmune & Allergic Diseases - CAAD University of Piemonte Orientale Corso Trieste 15/A, 28100, Novara, Italy Tel. 3334722270
contact emailmarcello.manfredi@uniupo.it
lab head
Marcello Manfredi
contact affiliationUniversity of Eastern Piedmont
contact emailmarcello.manfredi@uniupo.it
dataset submitter
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Dataset FTP location
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