PXD046838
PXD046838 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Trinucleotide cap analogs with N6-adenosine modifications: interaction partners in HEK293F cell line |
| Description | A series of trinucleotide cap analogs functionalized with an amine-terminated linker at the guanosine ribose and immobilized on BrCN-activated Sepharose was prepared. These affinity resins AR-1 (Am), AR-2 (m6Am), and AR-3 (Bn6Am) were incubated with HEK293F cell extract in the presence of GTP to limit non-specific interactions. The pulled-down proteins were eluted with the corresponding trinucleotide cap analog (m7GpppAmpG for AR-1, m7Gpppm6AmpG for AR-2, and m7GpppBn6AmpG for AR-3), digested with trypsin, labeled with isobaric tags (TMT), and analyzed by shotgun proteomics. This experiment was performed in order to assess binding preferences of proteins to the resins AR-1, AR-2, and AR-3. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_06:32:33.153.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Remigiusz Serwa |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-11-10 00:50:18 | ID requested | |
| 1 | 2024-03-08 01:44:29 | announced | |
| ⏵ 2 | 2024-10-22 06:32:34 | announced | 2024-10-22: Updated project metadata. |
Publication List
| Warminski M, Trepkowska E, Smietanski M, Sikorski PJ, Baranowski MR, Bednarczyk M, Kedzierska H, Majewski B, Mamot A, Papiernik D, Popielec A, Serwa RA, Shimanski BA, Sklepkiewicz P, Sklucka M, Sokolowska O, Spiewla T, Toczydlowska-Socha D, Warminska Z, Wolosewicz K, Zuberek J, Mugridge JS, Nowis D, Golab J, Jemielity J, Kowalska J, Mark Facilitates mRNA Purification and Confers Superior Translational Properties In Vitro and In Vivo. J Am Chem Soc, 146(12):8149-8163(2024) [pubmed] |
| 10.1021/jacs.3c12629; |
Keyword List
| submitter keyword: 5′ cap, N6-benzyladenosine,mRNA, HEK293, N6-methyladenosine |
Contact List
| Jacek Jemielity | |
|---|---|
| contact affiliation | Centre of New Technologies, University of Warsaw, 02-089 Warsaw, Poland |
| contact email | j.jemielity@cent.uw.edu.pl |
| lab head | |
| Remigiusz Serwa | |
| contact affiliation | The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences |
| contact email | r.serwa@imol.edu.pl |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD046838 |
| PRIDE project URI |
Repository Record List
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