PXD046828

PXD046828 is an original dataset announced via ProteomeXchange.

Title	The extracellular matrix protein signatures of the outer and inner zone of the rat adrenal cortex
Description	Different sites and changes in the tissue microenvironment have been associated with the cortex of adrenal gland functionality, maintenance, and renewal. Here we describe the differences between the extracellular matrix (ECM) signatures of the outer (OF) and inner fractions (IF) of the rat adrenal cortex. We compared the proteomic profiles of decellularized OF and IF rat adrenal samples followed by the analysis of the proteomic profiles of male and female samples. Our analysis of OF and IF samples simultaneously showed a total of 186 proteins, 69 proteins were identified in OF and 144 in IF. Ninety-two proteins were in common to both fractions, 5 proteins were exclusive to OF, and 89 proteins were exclusive to IF. A total of 42 proteins were assigned as ECM, 13 as structural ECM constituents, 17 as ECM components, and 12 as related to functional processes in ECM. Col14a1, Fbn2, and Ltbp4 were exclusive to and upregulated in the OF while Gpt2, Chga, Chgb, and Plg were exclusive to and upregulated in the IF. In addition, Nid2 and Lama2, both ECM components, were identified as upregulated in female OF and male IF adrenal, respectively. Next, we validated the main findings by using qPCR, histochemistry, and immunohistochemistry approaches.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:53:44.816.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Veronica Santiago
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-09 15:14:53	ID requested
1	2024-08-09 05:06:13	announced
⏵ 2	2024-10-22 06:53:48	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.4c00071;

Kremer JL, Santiago VF, Bongiovani Rodrigues F, Auricino TB, Freitas DHO, Palmisano G, Lotfi CFP, Extracellular Matrix Protein Signatures of the Outer and Inner Zones of the Rat Adrenal Cortex. J Proteome Res, 23(8):3418-3432(2024) [pubmed]

submitter keyword: adrenal cortex, rat,Extracellular matrix, adrenal gland

Claudimara Ferini
contact affiliation	Laboratory of Cellular Structure and Function, Department of Anatomy; Institute of Biomedical Science; University of São Paulo, São Paulo, Brazil.
contact email	clotfi@usp.br
lab head
Veronica Santiago
contact affiliation	University of Southampton
contact email	veronicafeijoli@usp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046828
     1. Label: PRIDE project
     2. Name: The extracellular matrix protein signatures of the outer and inner zone of the rat adrenal cortex