PXD046817

PXD046817 is an original dataset announced via ProteomeXchange.

Title	YAP modulates mitochondrial function and sensitive to NAMPT inhibition in triple negative breast cancer cells
Description	YAP and TAZ coordinate several NAD+-dependent processes like glycolysis, fatty acid oxidation and glutaminolysis in nutrient-deprivation conditions. However, correlations between NAD(H) levels and YAP/TAZ metabolic function are still poorly understood. We showed that stable silencing of YAP in the triple-negative breast cancer (TNBC) cell line MDAMB231 (MDA shYAP) rescued the toxicity of the NAD+ depletion agent FK866, both in 2D and in 3D culturing, prevented ROS accumulation and apoptosis. As MDA shYAP cells phenocopy the behavior of FK866-resistant MDAMB231 cells (RES), a comparative proteomic study between these cells lines was performed. We identified a deregulation of cell cycle in both MDA shYAP and RES, which prevents apoptosis initiation upon FK866 treatment. Moreover, we found an upregulation of mitochondrial proteins, in line with an increased mitochondrial biogenesis, function and mass, as previously observed for RES. Overall, we showed a YAP-dependent rewiring of mitochondrial metabolism and cell cycle checkpoints, which sustains the acquirement of resistance to FK866 in TNBC.
HostingRepository	PRIDE
AnnounceDate	2025-02-24
AnnouncementXML	Submission_2025-02-24_01:45:32.615.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046817
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Romina Belli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-09 07:09:53	ID requested
⏵ 1	2025-02-24 01:45:32	announced

10.6019/PXD046817;

submitter keyword: Mitochondrial fitness, Metabolic adaptation,YAP, Drug resistance, FK866, cell cycle, NAD(H)

Provenzani Alessandro
contact affiliation	University of Trento Department of Cellular, Computational and Integrative Biology - CIBIO University of Trento, Via Sommarive 9, I - 38123 Povo (Trento)
contact email	alessandro.provenzani@unitn.it
lab head
Romina Belli
contact affiliation	University of Trento
contact email	romina.belli@unitn.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046817
     1. Label: PRIDE project
     2. Name: YAP modulates mitochondrial function and sensitive to NAMPT inhibition in triple negative breast cancer cells