PXD046807

PXD046807 is an original dataset announced via ProteomeXchange.

Title	Super-SILAC-based quantitative proteomic analysis of lung adenocarcinoma associated with stage and EGFR gene status
Description	Lung adenocarcinoma (LUAD) is one of the most common causes of cancer death worldwide. Epidermal growth factor receptor (EGFR) mutations (MT) frequently target LUAD patients in Eastern Asia. EGFR tyrosine kinase inhibitors were developed to benefit LUAD patients harboring EGFR-activating MT. Unfortunately, biomarkers for early diagnosis and targeted therapy for patients with wild-type (WT) EGFR are currently lacking. Based on the super-SILAC, this study established a LUAD proteomics data set related to stage and EGFR status. Specifically, proteins obtained from three locally established LUAD cell lines were pooled with LUAD tissues from women, never smokers, different histological stages (early and late), and EGFR status (wild-type and mutant) to establish a quantitative proteome data set. We integrated genomic datasets and proteomic resources to select marker candidates for further characterization via immunohistochemistry and ELISA assays. The biological significance of marker candidates was examined using cell lines. This study provides new insights into the diagnosis and tumorigenesis of LUAD with EGFR-WT.
HostingRepository	PRIDE
AnnounceDate	2025-06-05
AnnouncementXML	Submission_2025-06-05_06:25:29.715.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chia-Jung Yu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-09 01:15:52	ID requested
⏵ 1	2025-06-05 06:25:30	announced

Dataset with its publication pending

submitter keyword: lung adenocarcinoma, tissue proteome, super-SILAC, EGFR, cancer stage

Chia-Jung Yu
contact affiliation	Department of Cell and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan
contact email	yucj1124@mail.cgu.edu.tw
lab head
Chia-Jung Yu
contact affiliation	Department of Cell and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan
contact email	yucj1124@mail.cgu.edu.tw
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD046807

PRIDE project URI

• PRIDE
  1. PXD046807
     1. Label: PRIDE project
     2. Name: Super-SILAC-based quantitative proteomic analysis of lung adenocarcinoma associated with stage and EGFR gene status