PXD046803

PXD046803 is an original dataset announced via ProteomeXchange.

Title	Ewing sarcoma immunopeptidomics
Description	Ewing Sarcoma (EwS) is a rare pediatric malignancy characterized by a unique t(11:22) (q24;q12) translocation resulting in the EWSR1::FLI1 fusion. Recent reports indicate the EWSR1::FLI1 fusion onco-protein drives aberrant expression of numerous transcripts, including Lipoxygenase Homology Domains 1 (LOX¬HD1). Given its highly restricted protein expression pattern and role in EwS tumorigenesis and metastasis, LOXHD1 may serve as a novel immunotherapeutic target in this malignancy. LOXHD1 immunogenic epitopes restricted to HLA-A*02:01 allowed for the isolation of high avidity TCRs. LOXHD1-specific TCR engineered CD8+ T cells confer cytotoxic activity against a panel of HLA-A*02:01+ EwS tumor cell lines and adoptive transfer leads to tumor eradication in a mouse xenograft model of EwS. This study nominates LOXHD1 as an onco-fusion regulated / non-mutated EwS tumor associated antigen with expression limited to inner hair cells of the cochlea, adult testis, and EwS
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_12:01:07.210.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046803
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pouya Faridi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-08 19:32:54	ID requested
⏵ 1	2025-05-06 12:01:08	announced

10.1038/s41598-025-96877-9;

Blanchard T, Faridi P, Xu C, Bear AS, Rasool RU, Huang G, Lim TCC, Ayala R, Gabunia K, Ji M, Posey AD, Scholler J, Asangani IA, Purcell AW, Linette GP, June CH, Carreno BM, LOXHD1 is an oncofusion-regulated antigen of ewing sarcoma. Sci Rep, 15(1):13007(2025) [pubmed]

10.6019/PXD046803;

submitter keyword: Ewing sarcoma, IFN gamma, Proteomics, Immunopeptidomics

Anthony Purcell
contact affiliation	Deputy Head (Research), Department of Biochemistry and Molecular Biology Head Immunoproteomics Laboratory Infection and Immunity Program Room 214, level 2, 15 Innovation Walk Monash Biomedicine Discovery Institute Monash University, Clayton Campus Clayton 3800 Victoria Australia
contact email	anthony.purcell@monash.edu
lab head
Pouya Faridi
contact affiliation	Monash University
contact email	pouya.faridi@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD046803

PRIDE project URI

• PRIDE
  1. PXD046803
     1. Label: PRIDE project
     2. Name: Ewing sarcoma immunopeptidomics