PXD046796

PXD046796 is an original dataset announced via ProteomeXchange.

Title	Probing Blood Plasma Protein Glycosylation with Infrared Spectroscopy
Description	The health state of an individual is closely linked to the glycosylation patterns of their blood plasma proteins. However, obtaining this information requires cost- and time-efficient analytical methods. We put forward infrared spectroscopy, which allows label-free analysis of protein glycosylation, but so far has only been applied to analyses of individual proteins. Although spectral information does not directly provide the molecular structure of the glycans, it is sensitive to changes therein and covers all types of glycosidic linkages. Combining single-step ion exchange chromatography with infrared spectroscopy, we developed a workflow that enables separation and analysis of major protein classes in blood plasma. Our results demonstrate that infrared spectroscopy can identify different patterns and global levels of glycosylation of intact plasma proteins. To showcase the strengths and limitations of the proposed approach, we compare the glycoforms of human and bovine alpha-1-acid glycoproteins, which exhibit highly variable global levels of glycosylation. To further independently evaluate our conclusions, the glycan moieties of human alpha-1-acid glycoprotein were further analyzed using established glycomics workflow. Importantly, the chromatographic separation of blood plasma improves the detection of aberrant glycoforms of a given protein, as compared to infrared spectroscopy of bulk plasma. The presented approach allows time-efficient comparison of glycosylation patterns of multiple plasma proteins, opening new avenues for biomedical probing.
HostingRepository	PRIDE
AnnounceDate	2024-02-26
AnnouncementXML	Submission_2024-02-26_10:14:32.606.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-08 15:04:09	ID requested
⏵ 1	2024-02-26 10:14:33	announced

Voronina L, Fleischmann F, Š, imunovi, ć J, Ludwig C, Novokmet M, Ž, igman M, Probing Blood Plasma Protein Glycosylation with Infrared Spectroscopy. Anal Chem, 96(7):2830-9(2024) [pubmed]

10.1021/acs.analchem.3c03589;

submitter keyword: glycosylation, ion exchange chromatography, proteomics, infrared spectroscopy, plasma,glycoproteins

Christina Ludwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS) Technical University Munich (TUM) Gregor-Mendel-Strasse 4 85354 Freising GERMANY
contact email	tina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046796
     1. Label: PRIDE project
     2. Name: Probing Blood Plasma Protein Glycosylation with Infrared Spectroscopy